Biochar immobilized plant growth-promoting rhizobacteria enhanced the physicochemical properties, agronomic characters and microbial communities during lettuce seedling

Frontiers in Microbiology

Microbiology

Ying-Yan Han¹  Qing-Jun Chen¹  Hui-Wen Yan¹  Guo-Qing Zhang¹  Ti-Kun Guan¹  Chao-Jie Liu¹  Jia-Shu Li¹  Qiu-Ying Wang²  Ya-Jie Zou³  Jian Sun⁴ 

[1] Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China;Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China;College of Horticulture, Xinjiang Agricultural University, Urumqi, China;Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China;Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China;

关键词: spent mushroom substrate;    biochar;    plant growth-promoting rhizobacteria;    microbial community;    lettuce seedling;   

DOI  :  10.3389/fmicb.2023.1218205

 received in 2023-05-06, accepted in 2023-06-19,  发布年份 2023

来源: Frontiers

【 摘 要 】

Spent mushroom substrate (SMS) is the by-products of mushroom production, which is mainly composed of disintegrated lignocellulosic biomass, mushroom mycelia and some minerals. The huge output and the lack of effective utilization methods make SMS becoming a serious environmental problem. In order to improve the application of SMS and SMS derived biochar (SBC), composted SMS (CSMS), SBC, combined plant growth-promoting rhizobacteria (PGPR, Bacillus subtilis BUABN-01 and Arthrobacter pascens BUAYN-122) and SBC immobilized PGPR (BCP) were applied in the lettuce seedling. Seven substrate treatments were used, including (1) CK, commercial control; (2) T1, CSMS based blank control; (3) T2, T1 with combined PGPR (9:1, v/v); (4) T3, T1 with SBC (19:1, v/v); (5) T4, T1 with SBC (9:1, v/v); (6) T5, T1 with BCP (19:1, v/v); (7) T6, T1 with BCP (9:1, v/v). The physicochemical properties of substrate, agronomic and physicochemical properties of lettuce and rhizospheric bacterial and fungal communities were investigated. The addition of SBC and BCP significantly (p < 0.05) improved the total nitrogen and available potassium content. The 5% (v/v) BCP addiction treatment (T5) represented the highest fresh weight of aboveground and underground, leave number, chlorophyll content and leaf anthocyanin content, and the lowest root malondialdehyde content. Moreover, high throughput sequencing revealed that the biochar immobilization enhanced the adaptability of PGPR. The addition of PGPR, SBC and BCP significantly enriched the unique bacterial biomarkers. The co-occurrence network analysis revealed that 5% BCP greatly increased the network complexity of rhizospheric microorganisms and improved the correlations of the two PGPR with other microorganisms. Furthermore, microbial functional prediction indicated that BCP enhanced the nutrient transport of rhizospheric microorganisms. This study showed the BCP can increase the agronomic properties of lettuce and improve the rhizospheric microbial community.

【 授权许可】

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Copyright © 2023 Guan, Wang, Li, Yan, Chen, Sun, Liu, Han, Zou and Zhang.