Development of a robust UPLC-MS/MS method for the quantification of riluzole in human plasma and its application in pharmacokinetics

Frontiers in Pharmacology

Pharmacology

Liying Cui¹  Bo Zhang²  Wei Zuo²  Tingting Xu²  Qiang Fu²  Xin Liu²  Zhuo Sun³  Ying Peng⁴ 

[1] Department of Neurology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;Department of Pharmacy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Beijing, China;Department of Pharmacy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Beijing, China;State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;

关键词: riluzole;    clobazam;    amyotrophic lateral sclerosis;    ultraperformance liquid chromatography-tandem mass spectrometry;    pharmacokinetics;   

DOI  :  10.3389/fphar.2023.1227354

 received in 2023-05-23, accepted in 2023-07-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: The aim of the present study was to establish a simple method for the determination of riluzole in human plasma by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and apply it for the determination of riluzole in amyotrophic lateral sclerosis (ALS) patients.Methods: Samples were prepared by protein precipitation and were then gradient-eluted on a column of ACQUITY UPLC® HSS T3 by using 0.1% formic acid acetonitrile and 0.1% formic acid water as the mobile phase. Detection was performed on a Xevo TQ-S tandem mass spectrometer in the multiple-reaction monitoring mode using positive electrospray ionization. Validation was performed in the range of 5–800 ng/mL.Results and discussion: Three batches of precision accuracy, selectivity, matrix effects, extraction recovery, and stability were also verified and met the requirements. The results showed that the method was reliable and successfully applied to the pharmacokinetics study of riluzole in Chinese amyotrophic lateral sclerosis patients. Meanwhile, in comparison with other prior published methods, our method has the advantages of simple sample preparation, relatively short running time, and small plasma sample consumption, which represented a high-throughput sample determination potential.

【 授权许可】

Unknown   
Copyright © 2023 Sun, Liu, Zuo, Fu, Xu, Cui, Zhang and Peng.