Metagenomic next-generation sequencing and proteomics analysis in pediatric viral encephalitis and meningitis

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Feng Gao¹  Yi Hua¹  Pei-Fang Jiang¹  Xiao-Tong Guo¹  Zhe-Feng Yuan¹  Xue-Bin Xu¹  Yi-Long Wang¹  Li-Hua Jiang¹  Meng-Ying Zhu¹  Xin Zhang¹  Guo-Xia Sheng¹  Yu-Chen Mao¹  Lu Xu¹  Cong-Ying Zhao¹ 

[1] Department of Neurology, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China;Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center For Child Health, Hangzhou, Zhejiang, China;

关键词: viral encephalitis (VE);    viral meningitis (VM);    human enterovirus (HEV);    metagenomic next-generation sequencing (mNGS);    MS-based proteomics;    diagnostic biomarkers;   

DOI  :  10.3389/fcimb.2023.1104858

 received in 2022-11-22, accepted in 2023-03-22,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionEarly and accurate identification of pathogens is essential for improved outcomes in patients with viral encephalitis (VE) and/or viral meningitis (VM).MethodsIn our research, Metagenomic next-generation sequencing (mNGS) which can identify viral pathogens unbiasedly was performed on RNA and DNA to identify potential pathogens in cerebrospinal fluid (CSF) samples from 50 pediatric patients with suspected VEs and/or VMs. Then we performed proteomics analysis on the 14 HEV-positive CSF samples and another 12 CSF samples from health controls (HCs). A supervised partial least squaresdiscriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) model was performed using proteomics data.ResultsTen viruses in 48% patients were identified and the most common pathogen was human enterovirus (HEV) Echo18. 11 proteins overlapping between the top 20 DEPs in terms of P value and FC and the top 20 proteins in PLS-DA VIP lists were acquired.DiscussionOur result showed mNGS has certain advantages on pathogens identification in VE and VM and our research established a foundation to identify diagnosis biomarker candidates of HEV-positive meningitis based on MS-based proteomics analysis, which could also contribute toward investigating the HEV-specific host response patterns.

【 授权许可】

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Copyright © 2023 Wang, Guo, Zhu, Mao, Xu, Hua, Xu, Jiang, Zhao, Zhang, Sheng, Jiang, Yuan and Gao