Fluorescence activated cell sorting and fermentation analysis to study rumen microbiome responses to administered live microbials and yeast cell wall derived prebiotics

Frontiers in Microbiology

Microbiology

Meiying Li¹  Greta Reintjes²  Lharbi Dridi³  Bastien Castagner³  Xiaohui Xing⁴  Long Jin⁴  Trevor W. Alexander⁴  Leeann Klassen⁴  D. Wade Abbott⁴  Carolyn Amundsen⁴ 

[1] College of Food Science, South China Agricultural University, Guangzhou, China;Department of Pharmacology & Therapeutics, Max Planck Institute for Marine Microbiology, Bremen, Germany;Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada;Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada;

关键词: rumen;    microbiome;    Bacteroides;    mannan;    yeast cell wall;    fermentation;    glycomics;   

DOI  :  10.3389/fmicb.2022.1020250

 received in 2022-08-16, accepted in 2022-12-12,  发布年份 2023

来源: Frontiers

【 摘 要 】

Rapid dietary changes, such as switching from high-forage to high-grain diets, can modify the rumen microbiome and initiate gastrointestinal distress, such as bloating. In such cases, feed additives, including prebiotics and live microbials, can be used to mitigate these negative consequences. Bio-Mos® is a carbohydrate-based prebiotic derived from yeast cells that is reported to increase livestock performance. Here, the responses of rumen bacterial cells to Bio-Mos® were quantified, sorted by flow cytometry using fluorescently-labeled yeast mannan, and taxonomically characterized using fluorescence in situ hybridization and 16S rRNA sequencing. Further, to evaluate the effects of bovine-adapted Bacteroides thetaiotaomicron administration as a live microbial with and without Bio-Mos® supplementation, we analyzed microbial fermentation products, changes to carbohydrate profiles, and shifts in microbial composition of an in vitro rumen community. Bio-Mos® was shown to be an effective prebiotic that significantly altered microbial diversity, composition, and fermentation; while addition of B. thetaiotaomicron had no effect on community composition and resulted in fewer significant changes to microbial fermentation. When combined with Bio-Mos®, there were notable, although not significant, changes to major bacterial taxa, along with increased significant changes in fermentation end products. These data suggest a synergistic effect is elicited by combining Bio-Mos® and B. thetaiotaomicron. This protocol provides a new in vitro methodology that could be extended to evaluate prebiotics and probiotics in more complex artificial rumen systems and live animals.

【 授权许可】

Unknown   
Copyright © 2023 Greta Reintjes, Meiying Li, Lharbi Dridi, Bastien Castagner, and His Majesty the King in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada for the contribution of Leeann Klassen, Long Jin, Carolyn Amundsen, Xiaohui Xing, Trevor W. Alexander, and D. Wade Abbott.