Anatomy of hypothalamic and diencephalic nuclei involved in seasonal fertility regulation in ewes

Frontiers in Veterinary Science

Veterinary Science

Carlos Palacios¹  Miguel Merchán²  Ignacio Plaza³  José Alfonso Abecia⁴  Rafael Coveñas⁵ 

[1] Animal Production Area, Department of Construction and Agronomy, Faculty of Agricultural and Environmental Sciences, University of Salamanca, Salamanca, Spain;Animal Production Area, Department of Construction and Agronomy, Faculty of Agricultural and Environmental Sciences, University of Salamanca, Salamanca, Spain;Laboratory of Neuroanatomy of the Peptidergic Systems, Institute for Neuroscience of Castilla y León (INCYL), University of Salamanca, Salamanca, Spain;Recognized Research Group - Molecular Bases of Development (Grupo de Investigación Reconocido - Bases Moleculares del Desarrollo - GIR-BMD), University of Salamanca, Salamanca, Spain;Auditory Neuroplasticity Laboratory, Institute for Neuroscience of Castilla y León (INCYL), University of Salamanca, Salamanca, Spain;Environmental Science Institute (IUCA), University of Zaragoza, Zaragoza, Spain;Laboratory of Neuroanatomy of the Peptidergic Systems, Institute for Neuroscience of Castilla y León (INCYL), University of Salamanca, Salamanca, Spain;Recognized Research Group - Molecular Bases of Development (Grupo de Investigación Reconocido - Bases Moleculares del Desarrollo - GIR-BMD), University of Salamanca, Salamanca, Spain;

关键词: calbindin;    calretinin;    parvalbumin;    GFAP;    IBA1;    Nissl staining;   

DOI  :  10.3389/fvets.2023.1101024

 received in 2022-11-17, accepted in 2023-01-31,  发布年份 2023

来源: Frontiers

【 摘 要 】

In this study, we describe in detail the anatomy of nuclei involved in seasonal fertility regulation (SFR) in ewes. For this purpose, the intergeniculate leaflet of the visual thalamus, the caudal hypothalamic arcuate nucleus, and suprachiasmatic, paraventricular and supraoptic nuclei of the rostral hypothalamus were morphometrically and qualitatively analyzed in Nissl-stained serial sections, in the three anatomical planes. In addition, data were collected on calcium-binding proteins and cell phenotypes after immunostaining alternate serial sections for calretinin, parvalbumin and calbindin. For a complete neuroanatomical study, glial architecture was assessed by immunostaining and analyzing alternate sections for glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (IBA1). The results showed a strong microglial and astroglia reaction around the hypothalamic nuclei of interest and around the whole 3rd ventricle of the ewe brain. Moreover, we correlated cytoarchitectonic coordinates of panoramic serial sections with their macroscopic localization and extension in midline sagittal-sectioned whole brain to provide guidelines for microdissecting nuclei involved in SFR.

【 授权许可】

Unknown   
Copyright © 2023 Merchán, Coveñas, Plaza, Abecia and Palacios.