Protein adsorption by nanomechanical mass spectrometry: Beyond the real-time molecular weighting

Frontiers in Molecular Biosciences

Molecular Biosciences

Wei Li¹  Ivo Stachiv¹  Chih-Yun Kuo² 

[1] Department of Functional Materials, Institute of Physics, Czech Academy of Sciences, Prague, Czechia;Department of Neurology and Centre of Clinical Neuroscience, First Faculty of Medicine and General University Hospital in Prague, Charles University, Prague, Czechia;

关键词: protein adsorption;    molecule mechanical properties;    molecular weight;    protein detection;    mass spectrometry;    mass sensing;   

DOI  :  10.3389/fmolb.2022.1058441

 received in 2022-09-30, accepted in 2022-12-14,  发布年份 2023

来源: Frontiers

【 摘 要 】

During past decades, enormous progress in understanding the mechanisms of the intermolecular interactions between the protein and surface at the single-molecule level has been achieved. These advances could only be possible by the ongoing development of highly sophisticated experimental methods such as atomic force microscopy, optical microscopy, surface plasmon resonance, ellipsometry, quartz crystal microbalance, conventional mass spectrometry, and, more recently, the nanomechanical systems. Here, we highlight the main findings of recent studies on the label-free single-molecule (protein) detection by nanomechanical systems including those focusing on the protein adsorption on various substrate surfaces. Since the nanomechanical techniques are capable of detecting and manipulating proteins even at the single-molecule level, therefore, they are expected to open a new way of studying the dynamics of protein functions. It is noteworthy that, in contrast to other experimental methods, where only given protein properties like molecular weight or protein stiffness can be determined, the nanomechanical systems enable a real-time measurement of the multiple protein properties (e.g., mass, stiffness, and/or generated surface stress), making them suitable for the study of protein adsorption mechanisms. Moreover, we also discuss the possible future trends in label-free detection and analysis of dynamics of protein complexes with these nanomechanical systems.

【 授权许可】

Unknown   
Copyright © 2023 Stachiv, Kuo and Li.