Frontiers in Cellular and Infection Microbiology | |
Molecular characterization of invasive Neisseria meningitidis isolates collected in Lithuania (2009-2019) and estimation of serogroup B meningococcal vaccine 4CMenB and MenB-Fhbp coverage | |
Cellular and Infection Microbiology | |
Inga Ivaškevičienė1  Vilmantas Gėgžna2  Indrė Kučinskaitė-Kodzė3  Dovilė Stravinskienė3  Milda Plečkaitytė3  Rūta Plepytė3  Emilija Sereikaitė3  Aurelija Žvirblienė3  Aurelija Petrutienė4  | |
[1] Clinic of Children’s Diseases, Institute of Clinical Medicine, Faculty of Medicine, Vilnius University, Vilnius, Lithuania;Pediatric Center, Vilnius University Hospital Santaros Klinikos, Vilnius, Lithuania;Institute of Biosciences, Life Sciences Center, Vilnius University, Vilnius, Lithuania;Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania;Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania;Department of Bacteriology, National Public Health Surveillance Laboratory, Vilnius, Lithuania; | |
关键词: Neisseria meningitidis; serogroup B; invasive meningococcal disease; multilocus sequence typing; gMATS; MenDeVAR Index; vaccine coverage; | |
DOI : 10.3389/fcimb.2023.1136211 | |
received in 2023-01-02, accepted in 2023-02-06, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
Neisseria meningitidis causes invasive meningococcal disease (IMD), which is associated with significant mortality and long-term consequences, especially among young children. The incidence of IMD in Lithuania was among the highest in European Union/European Economic Area countries during the past two decades; however, the characterization of meningococcal isolates by molecular typing methods has not yet been performed. In this study, we characterized invasive meningococcal isolates (n=294) recovered in Lithuania from 2009 to 2019 by multilocus sequence typing (MLST) and typing of antigens FetA and PorA. The more recent (2017-2019) serogroup B isolates (n=60) were genotyped by analyzing vaccine-related antigens to evaluate their coverage by four-component (4CMenB) and two-component (MenB-Fhbp) vaccines using the genetic Meningococcal Antigen Typing System (gMATS) and Meningococcal Deduced Vaccine Antigen Reactivity (MenDeVAR) Index methods, respectively. The vast majority (90.5%) of isolates belonged to serogroup B. MLST revealed a predominance of clonal complex 32 (74.02%). Serogroup B strain P1.19,15: F4-28: ST-34 (cc32) accounted for 64.1% of IMD isolates. The overall level of strain coverage by the 4MenB vaccine was 94.8% (CI 85.9-98.2%). Most serogroup B isolates (87.9%) were covered by a single vaccine antigen, most commonly Fhbp peptide variant 1 (84.5% of isolates). The Fhbp peptides included in the MenB-Fhbp vaccine were not detected among the analyzed invasive isolates; however, the identified predominant variant 1 was considered cross-reactive. In total, 88.1% (CI 77.5-94.1) of isolates were predicted to be covered by the MenB-Fhbp vaccine. In conclusion, both serogroup B vaccines demonstrate potential to protect against IMD in Lithuania.
【 授权许可】
Unknown
Copyright © 2023 Sereikaitė, Plepytė, Petrutienė, Stravinskienė, Kučinskaitė-Kodzė, Gėgžna, Ivaškevičienė, Žvirblienė and Plečkaitytė
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202310101846525ZK.pdf | 1505KB | download |