Stem Cell Therapy in Dengue Virus-Infected BALB/C Mice Improves Hepatic Injury

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Faizal Abu Bakar¹  Rusheni Munisvaradass²  Zhong Sun²  Akon Higuchi³  Badr Alzahrani⁴  Pooi Ling Mok⁵  Rukman Awang Hamat⁶  S. Sakinah⁶  Narcisse Joseph⁶  Jia Bei Tong⁶  Seoh Wei Teh⁶  Sivan Padma Priya⁶  Hui-yee Chee⁶  S. Suresh Kumar⁷  Chenglong Xiong⁸  Xiaoyun Wu⁹  Guozhong He^1,10  Antony V. Samrot^1,11  Mahendran Maniam^1,12 

[1] Bioinformatics and Computational Biology, Malaysia Genome Institute, National Institute of Biotechnology Malaysia (NIBM), Kajang, Malaysia;Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Seri Kembangan, Malaysia;Department of Chemical and Materials Engineering, National Central University, Taoyuan City, Taiwan;R&D Center for Membrane Technology, Chung Yuan Christian University, Taoyuan City, Taiwan;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakakah, Saudi Arabia;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakakah, Saudi Arabia;Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Seri Kembangan, Malaysia;Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Seri Kembangan, Malaysia;Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Seri Kembangan, Malaysia;Centre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Chennai, India;Department of Medical Microbiology, School of Public Health, Fudan University, Shanghai, China;First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, China;Institute of Health, Kunming Medical University, Kunming, China;School of Bioscience, Faculty of Medicine, Bioscience and Nursing, MAHSA University, Jenjarom, Malaysia;Sultan Idris Education University, Tanjong Malim, Malaysia;

关键词: dengue infection;    stem cell therapy;    DENV 2;    next-generation sequencing;    hepatology;   

DOI  :  10.3389/fcell.2021.637270

 received in 2020-12-03, accepted in 2021-05-25,  发布年份 2021

来源: Frontiers

【 摘 要 】

Extensive clinical efforts have been made to control the severity of dengue diseases; however, the dengue morbidity and mortality have not declined. Dengue virus (DENV) can infect and cause systemic damage in many organs, resulting in organ failure. Here, we present a novel report showing a tailored stem-cell-based therapy that can aid in viral clearance and rescue liver cells from further damage during dengue infection. We administered a combination of hematopoietic stem cells and endothelial progenitor cells in a DENV-infected BALB/c mouse model and found that delivery of this cell cocktail had improved their liver functions, confirmed by hematology, histopathology, and next-generation sequencing. These stem and progenitor cells can differentiate into target cells and repair the damaged tissues. In addition, the regime can regulate endothelial proliferation and permeability, modulate inflammatory reactions, enhance extracellular matrix production and angiogenesis, and secrete an array of growth factors to create an enhanced milieu for cell reparation. No previous study has been published on the treatment of dengue infection using stem cells combination. In conclusion, dengue-induced liver damage was rescued by administration of stem cell therapy, with less apoptosis and improved repair and regeneration in the dengue mouse model.

【 授权许可】

Unknown   
Copyright © 2021 Sakinah, Priya, Mok, Munisvaradass, Teh, Sun, Alzahrani, Abu Bakar, Chee, Awang Hamat, He, Xiong, Joseph, Tong, Wu, Maniam, Samrot, Higuchi and Kumar.