Next-generation sequencing: what are the needs in routine clinical microbiology? A survey among clinicians involved in infectious diseases practice

Frontiers in Medicine

Medicine

Marie Hallin¹  Charlotte Martin²  Jean-Christophe Goffard³  Thomas Demuyser⁴  Charlotte Michel⁵  Pierre Smeesters⁶ 

[1] Centre for Environmental Health and Occupational Health, School of Public Health, University Hospital Brussels, Université libre de Bruxelles (ULB), Brussels, Belgium;Department of Infectious Diseases, Saint-Pierre University Hospital, Université libre de Bruxelles (ULB), Brussels, Belgium;Department of Internal Medicine, University Hospital Brussels, Université libre de Bruxelles (ULB), Brussels, Belgium;Department of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium;Department of Microbiology, LHUB-ULB, Université libre de Bruxelles (ULB), Brussels, Belgium;Department of Pediatrics, University Hospital Brussels, Université libre de Bruxelles (ULB), Brussels, Belgium;Department of Pediatrics, University of Melbourne, Melbourne, VIC, Australia;

关键词: Next-Generation Sequencing (NGS);    clinical microbiological diagnosis;    questionnaire;    survey;    microbiological diagnosis;   

DOI  :  10.3389/fmed.2023.1225408

 received in 2023-05-19, accepted in 2023-08-08,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundThe translation of Next-Generation Sequencing (NGS) from research to clinical microbiology is increasing rapidly, but its integration into routine clinical care struggles to catch-up. A challenge for clinical laboratories is that the substantial investments made in the required technologies and resources must meet both current and forthcoming needs.MethodsTo get a clinical perspective of these needs, we have sent a survey to infectious diseases clinicians of five hospitals, covering the following topics: NGS knowledge, expected syndromes and patients foreseen to benefit from NGS, and expected impact on antimicrobial prescription.ResultsAccording to clinicians, benefits of NGS are mostly expected in neurological and respiratory infections diagnostics.ConclusionA better dialog between microbiologists and clinicians about hopes and limits of NGS in microbiology may help identifying key investments needed for clinical laboratories, today and tomorrow.

【 授权许可】

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Copyright © 2023 Michel, Martin, Smeesters, Goffard, Demuyser and Hallin.