Kruppel homolog 1 modulates ROS production and antimicrobial peptides expression in shrimp hemocytes during infection by the Vibrio parahaemolyticus strain that causes AHPND

Frontiers in Immunology

Immunology

Xiuli Chen¹  Yongzhen Zhao¹  Zhongyang Lin²  Zhihong Zheng²  Shengkang Li²  Yueling Zhang²  Jude Juventus Aweya³  Shangjie Liu⁴  Zhou Zheng⁴ 

[1] Guangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, China;Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China;Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China;College of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian, China;Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, China;Department of Medical Laboratory and Department of Reproductive Medicine, Luohu Clinical College of Shantou University Medical College, Shantou University, Shantou, China;

关键词: Penaeus vannamei;    AHPND;    Kr-h1;    IMD/Relish;    antimicrobial peptides;    ROS;   

DOI  :  10.3389/fimmu.2023.1246181

 received in 2023-06-26, accepted in 2023-08-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Shrimp aquaculture has been seriously affected by acute hepatopancreatic necrosis disease (AHPND), caused by a strain of Vibrio parahaemolyticus that carries the Pir toxin plasmids (V. parahaemolyticus (AHPND)). In this study, the transcription factor, Kruppel homolog 1-like of Peneaus vannamei (PvKr-h1), was significantly induced in shrimp hemocytes after V. parahaemolyticus (AHPND) challenge, suggesting that PvKr-h1 is involved in shrimp immune response. Knockdown of PvKr-h1 followed by V. parahaemolyticus (AHPND) challenge increased bacterial abundance in shrimp hemolymph coupled with high shrimp mortality. Moreover, transcriptome and immunofluorescence analyses revealed that PvKr-h1 silencing followed by V. parahaemolyticus (AHPND) challenge dysregulated the expression of several antioxidant-related enzyme genes, such as Cu-Zu SOD, GPX, and GST, and antimicrobial peptide genes, i.e., CRUs and PENs, and reduced ROS activity and nuclear translocation of Relish. These data reveal that PvKr-h1 regulates shrimps’ immune response to V. parahaemolyticus (AHPND) infection by suppressing antioxidant-related enzymes, enhancing ROS production and promoting nuclei import of PvRelish to stimulate antimicrobial peptide genes expression.

【 授权许可】

Unknown   
Copyright © 2023 Zheng, Liu, Lin, Aweya, Zheng, Zhao, Chen, Li and Zhang