Factors that influence the activity of biomass-degrading enzymes in the presence of ionic liquids—a review

Frontiers in Energy Research

Energy Research

Athiyya Umar¹  Mica Cabrera¹  Brian W. Blankenship¹  Blake A. Simmons¹  Kenneth L. Sale²  Paul Wolski²  Ezinne C. Achinivu³ 

[1] Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, United States;Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States;Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, United States;Sandia National Laboratories, Livermore, CA, United States;Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, United States;Sandia National Laboratories, Livermore, CA, United States;Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States;

关键词: enzymes;    ionic liquids;    biorefineries;    cellulose;    lignin;    pretreament;    lignocellulose;    biomass;   

DOI  :  10.3389/fenrg.2023.1212719

 received in 2023-04-26, accepted in 2023-08-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Ionic liquids (ILs) are seen as a more sustainable alternative to volatile organic solvents that are accelerating innovations in many industries such as energy storage, separations, and bioprocessing. The ability to effectively deconstruct lignocellulosic biomass is a significant hurdle in the biorefining/bioprocessing industry and presents limitations towards the commercial production of bioproducts (such as biofuels, biomaterials, etc.). Certain ILs have been shown to promote effective lignin removal, cellulose recovery, and sugar yields from various biomass feedstocks such as corn stover, wheat straw, sugarcane bagasse, sorghum, switchgrass, miscanthus, poplar, pine, eucalyptus, and certain mixtures of municipal solid waste. However, these improvements are often counteracted by the limited biocompatibility of ILs, which results in an IL-induced reduction in enzyme activity and stability—an important downstream step in the conversion of biomass to biofuels/bioproducts. As a result, significant efforts have been made to discover and engineer compatible enzyme-IL systems and to improve our understanding on the effect that these ILs have on these systems. This review seeks to examine the impact of ionic liquids on enzymes involved in lignocellulosic biomass deconstruction, with a specific focus on their relevance in the context of pretreatment. Beyond presenting an overview of the ionic liquid pretreatment landscape, we outline the main factors that influence enzyme activity and stability in the presence of ILs This data is consolidated and analyzed to apply this body of knowledge towards new innovations that could lead to improvements in the processing of biomass to biofuels and bioproducts.

【 授权许可】

Unknown   
Copyright © 2023 Wolski, Blankenship, Umar, Cabrera, Simmons, Sale and Achinivu.