Mapping the functional anatomy and topography of the cardiac autonomic innervation for selective cardiac neuromodulation using MicroCT

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Lydia M. Zopf¹  Paul Slezak²  Patrick Heimel³  Gunpreet Oberoi⁴  Bettina Kronsteiner⁵  Francesco Moscato⁶  Wolfgang J. Weninger⁷  Anne M. Kramer⁸  Attila Kiss⁸  Bruno K. Podesser⁸ 

[1] AUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria;AUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria;Austrian Cluster for Tissue Regeneration, Vienna, Austria;AUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria;Austrian Cluster for Tissue Regeneration, Vienna, Austria;Karl Donath Laboratory for Hard Tissue and Biomaterial Research, University Dental Clinic Vienna, Vienna, Austria;Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria;Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria;Ludwig Boltzmann Institute for Cardiovascular Research, Vienna, Austria;Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria;Ludwig Boltzmann Institute for Cardiovascular Research, Vienna, Austria;Austrian Cluster for Tissue Regeneration, Vienna, Austria;Department of Anatomy, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria;Division of Anatomy, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria;Ludwig Boltzmann Institute for Cardiovascular Research, Vienna, Austria;

关键词: vagus nerve stimulation;    cardiovascular diseases;    fascicular anatomy;    selective cardiac neuromodulation;    microcomputed tomography;    3D rendering Kronsteiner et al. imaging of cardiac autonomic innervation;   

DOI  :  10.3389/fcell.2022.968870

 received in 2022-06-14, accepted in 2022-08-24,  发布年份 2022

来源: Frontiers

【 摘 要 】

Background: Vagus nerve stimulation (VNS) has gained great importance as a promising therapy for a myriad of diseases. Of particular interest is the therapy of cardiovascular diseases, such as heart failure or atrial fibrillation using selective cardiac VNS. However, there is still a lack of organ-specific anatomical knowledge about the fascicular anatomy and topography of the cardiac branch (CB), which diminishes the therapeutic possibilities for selective cardiac neuromodulation. Here, we established a topographical and anatomical map of the superior cardiac VN in two animal species to dissect cervical and cardiac VN morphology.Methods: Autonomic nerves including superior CBs were harvested from domestic pigs and New Zeeland rabbits followed by imaging with microcomputed tomography (µCT) and 3D rendering. The data were analyzed in terms of relevant topographical and anatomical parameters.Results: Our data showed that cardiac vagal fascicles remained separated from other VN fascicles up to 22.19 mm (IQR 14.02–41.30 mm) in pigs and 7.68 mm (IQR 4.06–12.77 mm) in rabbits from the CB point and then started merging with other fascicles. Exchanges of nerve fascicles between sympathetic trunk (ST) and VN were observed in 3 out of 11 nerves, which might cause additional unwanted effects in unselective VNS. Our 3D rendered digital model of the cardiac fascicles was generated showing that CB first remained on the medial side where it branched off the VN, as also shown in the µCT data of 11 pig nerves, and then migrated towards the ventromedial site the further it was traced cranially.Conclusion: Our data provided an anatomical map of the cardiac vagal branches including cervical VN and ST for future approaches of selective cardiac neurostimulation, indicating the best position of selective cardiac VNS just above the CB point.

【 授权许可】

Unknown   
Copyright © 2022 Kronsteiner, Zopf, Heimel, Oberoi, Kramer, Slezak, Weninger, Podesser, Kiss and Moscato.