Mobile genetic elements carrying aminoglycoside resistance genes in Acinetobacter baumannii isolates belonging to global clone 2

Frontiers in Microbiology

Microbiology

Arash Seifi¹  Abdolhossein Naseri²  Mohammad Rahbar³  Parisa Eslami⁴  Malihe Talebi⁵  Alireza Abdollahi⁶  Ghazal Naderi⁷  Roghayeh Gheybizadeh⁷  Sedigheh Ghourchian⁷  Masoumeh Douraghi⁷ 

[1] Department of Infectious Diseases, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran;Department of Laboratory Sciences, School of Paramedical Sciences, Iran University of Medical Sciences, Tehran, Iran;Department of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran;Department of Microbiology, Milad Hospital, Tehran, Iran;Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;Department of Pathology, Imam Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran;Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;

关键词: Acinetobacter baumannii;    aminoglycoside resistance;    global clone 2;    mobile genetic element (MGE);    plasmids;   

DOI  :  10.3389/fmicb.2023.1172861

 received in 2023-02-23, accepted in 2023-04-12,  发布年份 2023

来源: Frontiers

【 摘 要 】

Aminoglycosides are used to treat infections caused by carbapenem-resistant Acinetobacter baumannii (CRAB) strains. However, resistance to aminoglycosides has increased remarkably in the last few years. Here, we aimed to determine the mobile genetic elements (MGEs) associated with resistance to aminoglycosides in the global clone 2 (GC2) A. baumannii. Among the 315 A. baumannii isolates, 97 isolates were identified as GC2, and 52 of GC2 isolates (53.6%) were resistant to all the aminoglycosides tested. The AbGRI3s carrying armA were detected in 88 GC2 isolates (90.7%), and of them, 17 isolates (19.3%) carried a new variant of AbGRI3 (AbGRI3ABI221). aphA6 was located in TnaphA6 of 30 isolates out of 55 aphA6-harboring isolates, and 20 isolates were found to harbor TnaphA6 on a RepAci6 plasmid. Tn6020 carrying aphA1b was detected in 51 isolates (52.5%), which was located within AbGRI2 resistance islands. The pRAY* carrying the aadB gene was detected in 43 isolates (44.3%), and no isolate was found to contain a class 1 integron harboring this gene. The GC2 A. baumannii isolates contained at least one MGE carrying the aminoglycoside resistance gene, located mostly either in the chromosome within AbGRIs or on the plasmids. Thus, it is likely that these MGEs play a role in the dissemination of aminoglycoside resistance genes in GC2 isolates from Iran.

【 授权许可】

Unknown   
Copyright © 2023 Naderi, Talebi, Gheybizadeh, Seifi, Ghourchian, Rahbar, Abdollahi, Naseri, Eslami and Douraghi.