Frontiers in Veterinary Science | |
Surveillance for feline herpesvirus type 1 mutation and development of resistance in cats treated with antiviral medications | |
Veterinary Science | |
Morgan E. Marino1  Nikole E. Ineck1  Ugochi Emelogu1  Chin-Chi Liu1  Melanie A. Mironovich1  Erinn P. Mills1  Renee T. Carter1  Pilar Camacho-Luna1  Andrew C. Lewin2  | |
[1] Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, United States;null; | |
关键词: feline herpesvirus; cidofovir; famciclovir; ganciclovir; antiviral; resistance; mutation; genomics; | |
DOI : 10.3389/fvets.2023.1197249 | |
received in 2023-03-30, accepted in 2023-05-02, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
Feline herpesvirus type 1 (FHV-1) commonly causes ocular surface disease in cats and is treated with antiviral medications targeting viral DNA polymerase (UL30/42). Herein, we describe a method to assess the FHV-1 genome for mutation development and to assess the functional impact of mutations, if present. Fourteen shelter-housed domestic cats with FHV-1 ocular surface disease were assigned to one of four treatment groups: placebo (n = 3), cidofovir 0.5% ophthalmic solution (n = 3), famciclovir oral solution (n = 5), or ganciclovir 0.15% ophthalmic solution (n = 3). Swabs were collected before (day 1) and after (day 8) 1 week of twice-daily treatments to isolate viable FHV-1. Viral DNA was extracted for sequencing using Illumina MiSeq with subsequent genomic variant detection between paired day 1 and day 8 isolates. Plaque reduction assay was performed on paired isolates demonstrating non-synonymous variants. A total of 171 synonymous and 3 non-synonymous variants were identified in day 8 isolates. No variants were detected in viral UL23, UL30, or UL42 genes. Variant totals were not statistically different in animals receiving antiviral or placebo (p = 0.4997). A day 8 isolate from each antiviral treatment group contained a single non-synonymous variant in ICP4 (transcriptional regulator). These 3 isolates demonstrated no evidence of functional antiviral resistance when IC50 was assessed. Most (10/14 pairs) day 1 and 8 viral isolate pairs from the same host animal were near-identical. While functional variants were not detected in this small sample, these techniques can be replicated to assess FHV-1 isolates suspected of having developed resistance to antiviral medications.
【 授权许可】
Unknown
Copyright © 2023 Lewin, Ineck, Mironovich, Marino, Liu, Emelogu, Mills, Camacho-Luna and Carter.
【 预 览 】
Files | Size | Format | View |
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RO202310101354745ZK.pdf | 805KB | download |