Exploration and validation of the influence of angiogenesis-related factors in aortic valve calcification

Frontiers in Cardiovascular Medicine

Cardiovascular Medicine

KaiMing Wei¹  XiangJin Kong¹  FuShun Lin¹  ChuanZhen Liu¹  Xin Lv¹  LingWei Meng¹  XingHua Gu¹ 

[1] Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China;Department of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China;

关键词: calcific aortic valve disease (CAVD);    angiogenesis-related factors;    secretogranin II (SCG2);    zinc finger E-box binding homeobox 1 (ZEB1);    miRNA;    transcription factors (TFs);   

DOI  :  10.3389/fcvm.2023.1061077

 received in 2022-10-04, accepted in 2023-01-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

Over the years, bioinformatics tools have been used to identify functional genes. In the present study, bioinformatics analyses were conducted to explore the underlying molecular mechanisms of angiogenic factors in calcific aortic valve disease (CAVD). The raw gene expression profiles were from datasets GSE153555, GSE83453, and GSE51472, and the angiogenesis-related gene set was from the Gene Set Enrichment Analysis database (GSEA). In this study, R was used to screen for differentially expressed genes (DEGs) and co-expressed genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) Pathway enrichment analysis were performed on DEGs and validated in clinical samples. DEGs in CAVD were significantly enriched in numerous immune response pathways, inflammatory response pathways and angiogenesis-related pathways. Nine highly expressed angiogenesis-related genes were identified, of which secretogranin II (SCG2) was the most critical gene. MiRNA and transcription factors (TFs) networks were established centered on five DEGs, and zinc finger E-box binding homeobox 1 (ZEB1) was the most important transcription factor, verified by PCR, immunohistochemical staining and western blotting experiments. Overall, this study identified key genes and TFs that may be involved in the pathogenesis of CAVD and may have promising applications in the treatment of CAVD.

【 授权许可】

Unknown   
Copyright © 2023 Kong, Meng, Wei, Lv, Liu, Lin and Gu.