The rootstock shape microbial diversity and functionality in the rhizosphere of Vitis vinifera L. cultivar Falanghina

Frontiers in Plant Science

Plant Science

Giuseppe Marotta¹  Alessandra Falzarano²  Antonello Prigioniero²  Maria Antonietta Ranauda²  Maria Maisto²  Maria Tartaglia²  Rosaria Sciarrillo²  Daniela Zuzolo²  Carmine Guarino² 

[1] Department of Law, Economics, Management and Quantitative Methods (DEMM), University of Sannio, Benevento, Italy;Department of Science and Technologies, University of Sannio, Benevento, Italy;

关键词: rootstock;    grapevine;    microbial terroir;    rhizosphere;    holobiont;    microbial diversity;   

DOI  :  10.3389/fpls.2023.1205451

 received in 2023-04-13, accepted in 2023-07-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

The rhizosphere effect occurring at the root-soil interface has increasingly been shown to play a key role in plant fitness and soil functionality, influencing plants resilience. Here, for the first time, we investigated whether the rootstock genotype on which Vitis vinifera L. cultivar Falanghina is grafted can influence the rhizosphere microbiome. Specifically, we evaluated to which extent the 5BB and 1103P rootstocks are able to shape microbial diversity of rhizosphere environment. Moreover, we explored the potential function of microbial community and its shift under plant genotype influence. We investigated seven vineyards subjected to the same pedo-climatic conditions, similar age, training system and management and collected twelve rhizosphere soil samples for metagenomic analyses and composite soil samples for physical-chemical properties. In this study, we used 16S rRNA gene-based metagenomic analysis to investigate the rhizosphere bacterial diversity and composition. Liner discriminant analysis effect size (LEFSe) was conducted for metagenomic biomarker discovery. The functional composition of sampled communities was determined using PICRUSt, which is based on marker gene sequencing profiles. Soil analyses involved the determination of texture, pH, Cation Exchange Capacity (CSC), Organic Carbon (OC), electrical conductivity (EC), calcium (Ca), magnesium (Mg), potassium (K) content, Phosphorous (P), nitrogen (N). The latter revealed that soil features were quite homogenous. The metagenomic data showed that the bacterial alpha-diversity (Observed OTUs) significantly increased in 1103P rhizosphere microbiota. Irrespective of cultivar, Pseudomonadota was the dominant phylum, followed by Actinomycetota > Bacteroidota > Thermoproteota. However, Actinomycetota was the major marker phyla differentiating the rhizosphere microbial communities associated with the different rootstock types. At the genus level, several taxa belonging to Actinomycetota and Alphaproteobacteria classes were enriched in 1103P genotype rhizosphere. Investigating the potential functional profile, we found that most key enzyme-encoding genes involved in N cycling were significantly more abundant in 5BB rootstock rhizosphere soil. However, we found that 1103P rhizosphere was enriched in genes involved in C cycle and Plant Growth Promotion (PGP) functionality. Our results suggest that the different rootstocks not only recruit specific bacterial communities, but also specific functional traits within the same environment.

【 授权许可】

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Copyright © 2023 Zuzolo, Ranauda, Maisto, Tartaglia, Prigioniero, Falzarano, Marotta, Sciarrillo and Guarino