Integrated transcriptomic analysis identifies coordinated responses to nitrogen and phosphate deficiency in rice

Frontiers in Plant Science

Plant Science

Yijian Liu¹  Yunrong Wu²  Luying Ying²  Xiaorong Mo²  Yan Wang²  Zhongchang Wu²  Hong Lu²  Yu Liu²  Fei Wang²  Jiming Xu²  Chuanzao Mao³ 

[1] Hainan Institute, Zhejiang University, Yazhou Bay Science and Technology City, Sanya, Hainan, China;State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;Hainan Institute, Zhejiang University, Yazhou Bay Science and Technology City, Sanya, Hainan, China;

关键词: rice;    nitrogen;    phosphate;    interaction;    uptake;    transcriptome;    NIGT1;   

DOI  :  10.3389/fpls.2023.1164441

 received in 2023-02-12, accepted in 2023-03-15,  发布年份 2023

来源: Frontiers

【 摘 要 】

Nitrogen (N) and phosphorus (P) are two primary components of fertilizers for crop production. Coordinated acquisition and utilization of N and P are crucial for plants to achieve nutrient balance and optimal growth in a changing rhizospheric nutrient environment. However, little is known about how N and P signaling pathways are integrated. We performed transcriptomic analyses and physiological experiments to explore gene expression profiles and physiological homeostasis in the response of rice (Oryza sativa) to N and P deficiency. We revealed that N and P shortage inhibit rice growth and uptake of other nutrients. Gene Ontology (GO) analysis of differentially expressed genes (DEGs) suggested that N and Pi deficiency stimulate specific different physiological reactions and also some same physiological processes in rice. We established the transcriptional regulatory network between N and P signaling pathways based on all DEGs. We determined that the transcript levels of 763 core genes changed under both N or P starvation conditions. Among these core genes, we focused on the transcription factor gene NITRATE-INDUCIBLE, GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1 (NIGT1) and show that its encoded protein is a positive regulator of P homeostasis and a negative regulator of N acquisition in rice. NIGT1 promoted Pi uptake but inhibited N absorption, induced the expression of Pi responsive genes PT2 and SPX1 and repressed the N responsive genes NLP1 and NRT2.1. These results provide new clues about the mechanisms underlying the interaction between plant N and P starvation responses.

【 授权许可】

Unknown   
Copyright © 2023 Wang, Wang, Ying, Lu, Liu, Liu, Xu, Wu, Mo, Wu and Mao