Water extract of cacumen platycladi promotes hair growth through the Akt/GSK3β/β-catenin signaling pathway

Frontiers in Pharmacology

Pharmacology

Qingqing Mao¹  Jinyuan Liu¹  Hangjie Fu¹  Bin Ding¹  Zhiwei Weng¹  Zhiguang Huang¹  Wenxia Li² 

[1]College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China

[2]Academy of Chinese Medical Science, Zhejiang Chinese Medical University, Hangzhou, China

[3]The Fourth School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, China

关键词: alopecia;    cacumen platycladi;    hair follicles;    dermal papilla cells;    Akt;    GSK3β;   

DOI  :  10.3389/fphar.2023.1038039

 received in 2022-09-06, accepted in 2023-02-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Cacumen Platycladi (CP) consists of the dried needles of Platycladus orientalis L.) Franco. It was clinically demonstrated that it effectively regenerates hair, but the underlying mechanism remains unknown. Thus, we employed shaved mice to verify the hair growth-promoting capability of the water extract of Cacumen Platycladi (WECP). The morphological and histological analyses revealed that WECP application could significantly promote hair growth and hair follicles (HFs) construction, in comparison to that of control group. Additionally, the skin thickness and hair bulb diameter were significantly increased by the application of WECP in a dose-dependent manner. Besides, the high dose of WECP also showed an effect similar to that of finasteride. In an in vitro assay, WECP stimulated dermal papilla cells (DPCs) proliferation and migration. Moreover, the upregulation of cyclins (cyclin D1, cyclin-dependent kinase 2 (CDK2), and cyclin-dependent kinase 4 (CDK4)) and downregulation of P21 in WECP-treated cell assays have been evaluated. We identified the ingredients of WECP using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) and endeavored to predict their relevant molecular mechanisms by network analysis. We found that the Akt (serine/threonine protein kinase) signaling pathway might be a crucial target of WECP. It has been demonstrated that WECP treatment activated the phosphorylation of Akt and glycogen synthase kinase-3-beta (GSK3β), promoted β-Catenin and Wnt10b accumulation, and upregulated the expression of lymphoid enhancer-binding factor 1 (LEF1), vascular endothelial growth factor (VEGF), and insulin-like growth factor 1 (IGF1). We also found that WECP significantly altered the expression levels of apoptosis-related genes in mouse dorsal skin. The enhancement capability of WECP on DPCs proliferation and migration could be abrogated by the Akt-specific inhibitor MK-2206 2HCl. These results suggested that WECP might promote hair growth by modulating DPCs proliferation and migration through the regulation of the Akt/GSK3β/β-Catenin signaling pathway.

【 授权许可】

Unknown   
Copyright © 2023 Fu, Li, Weng, Huang, Liu, Mao and Ding.