Comparative genomic analysis of Cohnella hashimotonis sp. nov. isolated from the International Space Station

Frontiers in Microbiology

Microbiology

Anna C. Simpson¹  Ceth W. Parker¹  Kasthuri Venkateswaran¹  Nitin K. Singh¹  Namita Damle²  Christopher E. Mason²  V. V. Ramprasad Eedara³  Fathi Karouia⁴ 

[1] California Institute of Technology, Jet Propulsion Laboratory, Pasadena, CA, United States;Department of Physiology and Biophysics, and the WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY, United States;Department of Plant Science, School of Life Sciences, University of Hyderabad, Hyderabad, India;NASA Ames, Moffet Field, CA, United States;

关键词: International Space Station (ISS);    novel bacteria;    Cohnella;    space biology;    pangenome analysis;   

DOI  :  10.3389/fmicb.2023.1166013

 received in 2023-02-14, accepted in 2023-05-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

A single strain from the family Paenibacillaceae was isolated from the wall behind the Waste Hygiene Compartment aboard the International Space Station (ISS) in April 2018, as part of the Microbial Tracking mission series. This strain was identified as a gram-positive, rod-shaped, oxidase-positive, catalase-negative motile bacterium in the genus Cohnella, designated as F6_2S_P_1T. The 16S sequence of the F6_2S_P_1T strain places it in a clade with C. rhizosphaerae and C. ginsengisoli, which were originally isolated from plant tissue or rhizosphere environments. The closest 16S and gyrB matches to strain F6_2S_P_1T are to C. rhizosphaerae with 98.84 and 93.99% sequence similarity, while a core single-copy gene phylogeny from all publicly available Cohnella genomes places it as more closely related to C. ginsengisoli. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values to any described Cohnella species are <89 and <22%, respectively. The major fatty acids for strain F6_2S_P_1T are anteiso-C15:0 (51.7%), iso-C16:0 (23.1%), and iso-C15:0 (10.5%), and it is able to metabolize a wide range of carbon compounds. Given the results of the ANI and dDDH analyses, this ISS strain is a novel species within the genus Cohnella for which we propose the name Cohnella hashimotonis, with the type strain F6_2S_P_1T (=NRRL B-65657T and DSMZ 115098T). Because no closely related Cohnella genomes were available, this study generated the whole-genome sequences (WGSs) of the type strains for C. rhizosphaerae and C. ginsengisoli. Phylogenetic and pangenomic analysis reveals that F6_2S_P_1T, C. rhizosphaerae, and C. ginsengisoli, along with two uncharacterized Cohnella strains, possess a shared set of 332 gene clusters which are not shared with any other WGS of Cohnella species, and form a distinct clade branching off from C. nanjingensis. Functional traits were predicted for the genomes of strain F6_2S_P_1T and other members of this clade.

【 授权许可】

Unknown   
Copyright © 2023 Simpson, Eedara, Singh, Damle, Parker, Karouia, Mason and Venkateswaran.