Comparative assessment of the SjSAP4-incorporated gold immunochromatographic assay for the diagnosis of human schistosomiasis japonica

Frontiers in Public Health

Public Health

Remigio M. Olveda¹  Donald P. McManus²  Yi Mu²  Kosala G. Weerakoon²  Pengfei Cai³  Jonas Rivera³  Catherine A. Gordon⁴  Hong You⁵  Malcolm K. Jones⁵  Allen G. Ross⁶ 

[1] Department of Immunology, Research Institute for Tropical Medicine, Manila, Philippines;Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia;Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia;School of Biomedical Sciences, The University of Queensland, Brisbane, QLD, Australia;Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia;School of Public Health, Faculty of Medicine, The University of Queensland, Brisbane, QLD, Australia;Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia;School of Veterinary Science, The University of Queensland, Brisbane, QLD, Australia;Rural Health and Medical Research Institute, Charles Sturt University, Orange, NSW, Australia;

关键词: schistosomiasis;    Schistosoma japonicum;    gold immunochromatographic assay (GICA);    POC-CCA;    droplet digital PCR;    diagnosis;    SjSAP4;    ASSURED criteria;   

DOI  :  10.3389/fpubh.2023.1249637

 received in 2023-06-29, accepted in 2023-08-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundSchistosomiasis, a disease caused by parasites of the genus Schistosoma, remains a global public health threat. This study aimed to validate the diagnostic performance of a recently developed gold immunochromatographic assay (GICA) for the detection of S. japonicum infection in a rural endemic area of the Philippines.MethodsHuman clinical samples were collected from 412 subjects living in Laoang and Palapag municipalities, Northern Samar, the Philippines. The presence of Schistosoma-specific antibodies in serum samples was tested with the SjSAP4-incorporated GICA strips and the results were converted to fully quantitative data by introducing an R value. The performance of the established GICA was further compared with other diagnostic tools, including the Kato-Katz (KK) technique, point-of-care circulating cathodic antigen (POC-CCA), droplet digital (dd) PCR, and enzyme-linked immunosorbent assays (ELISAs).ResultsThe developed GICA strip was able to detect KK positive individuals with a sensitivity of 83.3% and absolute specificity. When calibrated with the highly sensitive faecal ddPCR assay, the immunochromatographic assay displayed an accuracy of 60.7%. Globally, the GICA assay showed a high concordance with the SjSAP4-ELISA assay. The schistosomiasis positivity rate determined by the GICA test was similar to those obtained with the SjSAP4-ELISA assay and the ddPCR assay performed on serum samples (SR_ddPCR), and was 2.3 times higher than obtained with the KK method.ConclusionThe study further confirms that the developed GICA is a valuable diagnostic tool for detecting light S. japonicum infections and implies that this point-of-care assay is a viable solution for surveying endemic areas of low-intensity schistosomiasis and identifying high-priority endemic areas for targeted interventions.

【 授权许可】

Unknown   
Copyright © 2023 Mu, Rivera, McManus, Weerakoon, Ross, Olveda, Gordon, You, Jones and Cai.