Mycelium-based biomaterials as smart devices for skin wound healing

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Dalila Miele¹  Barbara Vigani¹  Silvia Rossi¹  Giuseppina Sandri¹  Marco Ruggeri¹  Cinzia Boselli¹  Antonia Icaro Cornaglia²  Marco Contardi³  Athanassia Athanassiou³  Giulia Suarato³ 

[1] Department of Drug Sciences, University of Pavia, Pavia, Italy;Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, Italy;Smart Materials, Istituto Italiano di Tecnologia, Genova, Italy;

关键词: mycelium;    Ganoderma lucidum;    Pleurotus ostreatus;    biocompatibility;    gene expression;    murine model;   

DOI  :  10.3389/fbioe.2023.1225722

 received in 2023-05-19, accepted in 2023-08-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Recently, mycelia of Ganoderma lucidum and Pleurotus ostreatus, edible fungi, have been characterized in vitro as self-growing biomaterials for tissue engineering since they are constituted of interconnected fibrous networks resembling the dermal collagen structure.Aim: This work aims to investigate the biopharmaceutical properties of G. lucidum and P. ostreatus mycelia to prove their safety and effectiveness in tissue engineering as dermal substitutes.Methods: The mycelial materials were characterized using a multidisciplinary approach, including physicochemical properties (morphology, thermal behavior, surface charge, and isoelectric point). Moreover, preclinical properties such as gene expression and in vitro wound healing assay have been evaluated using fibroblasts. Finally, these naturally-grown substrates were applied in vivo using a murine burn/excisional wound model.Conclusions: Both G. lucidum and P. ostreatus mycelia are biocompatible and able to safely and effectively enhance tissue repair in vivo in our preclinical model.

【 授权许可】

Unknown   
Copyright © 2023 Ruggeri, Miele, Contardi, Vigani, Boselli, Icaro Cornaglia, Rossi, Suarato, Athanassiou and Sandri.