Commercial production of Florida pompano (Trachinotus carolinus) larvae at low salinity induces variable changes in whole-larvae microbial diversity, gene expression, and gill histopathology

Frontiers in Marine Science

Marine Science

Laura E. King¹  David J. Bradshaw¹  Paul S. Wills¹  Marty Riche¹  Sahar Mejri¹  Carlie S. Perricone¹  Elizabeth B. Allmon²  Maria Sepúlveda³  Nicole Kirchhoff⁴ 

[1] Department of Aquaculture and Stock Enhancement, Florida Atlantic University at Harbor Branch Oceanographic Institute, Fort Pierce, FL, United States;Department of Forestry and Natural Resources, Purdue University, West Lafayette, IN, United States;Department of Forestry and Natural Resources, Purdue University, West Lafayette, IN, United States;Sustainability Research Center & PhD in Conservation Medicine, Life Sciences Faculty, Universidad Andres Bello, Santiago, Chile;Live Advantage Bait, LLC, Fort Pierce, FL, United States;

关键词: Trachinotus carolinus;    salinity;    microbiome;    whole transcriptome;    gill histopathology;    Vibrio;    Flavobacterium;    immunology;   

DOI  :  10.3389/fmars.2023.1158446

 received in 2023-02-03, accepted in 2023-03-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionSalinity presents economic and technical challenges in land-based recirculating aquaculture systems (RAS) in the U.S. warm water marine finfish aquaculture industry. Many studies have shown euryhaline fish reared at salinities closer to their iso-osmotic salinity can yield enhanced production performance as well as potential reduced costs to farms. However, there is potential for osmotic stress in fish larvae to negatively impact larvae microbiome and innate immune system. Florida pompano (Trachinotus carolinus) is a popular sportfish has been targeted for land-based RAS due to its impressive market value and euryhaline capacity. This study investigated the impacts of rearing Florida pompano larvae at salinities closer to their iso-osmotic salinity.Materials and methodsLarvae were cultured at 10, 20, and 30 ppt in triplicates, and larvae samples were collected for histopathology, microbiome, and whole transcriptomics analysis every three days from hatching until the time of weaning (24 days post hatch [DPH]). Water samples were also taken for microbiome analysis on every other larval sampling day. DiscussionThese changes were driven more by metamorphosis, causing an increase in expression of antioxidant genes (cat, gss, gsto1, and scara3) than by the presence of potentially pathogenic genera, which failed to induce an immune response (low or unchanged expression of downstream elements of the NOD1 or TLR5 pathways). These findings provide baseline information on Florida pompano low salinity tolerance in larviculture during early developmental stages. In addition, we have shown minimal effects on the immune system at salinities as low as 10 ppt. This work has important implications for larval health management and can be used to refine and direct future research regarding improving commercial production of warm water marine species

【 授权许可】

Unknown   
Copyright © 2023 Bradshaw, Perricone, King, Allmon, Sepúlveda, Riche, Wills, Kirchhoff and Mejri