Detection of blaCTX-M and blaDHA genes in stool samples of healthy people: comparison of culture- and shotgun metagenomic-based approaches

Frontiers in Microbiology

Microbiology

Parham Sendi¹  Aline I. Moser¹  Claudia Aldeia¹  Andrea Endimiani¹  Edgar I. Campos-Madueno²  Vincent Perreten³ 

[1] Institute for Infectious Diseases (IFIK), University of Bern, Bern, Switzerland;Institute for Infectious Diseases (IFIK), University of Bern, Bern, Switzerland;Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland;Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland;

关键词: ESBL;    AmpC;    stool;    metagenomics;    Illumina;    enrichment;   

DOI  :  10.3389/fmicb.2023.1236208

 received in 2023-06-07, accepted in 2023-08-16,  发布年份 2023

来源: Frontiers

【 摘 要 】

We implemented culture- and shotgun metagenomic sequencing (SMS)-based methods to assess the gut colonization with extended-spectrum cephalosporin-resistant Enterobacterales (ESC-R-Ent) in 42 volunteers. Both methods were performed using native and pre-enriched (broth supplemented with cefuroxime) stools. Native culture screening on CHROMID® ESBL plates resulted in 17 positive samples, whereas the pre-enriched culture (gold-standard) identified 23 carriers. Overall, 26 ESC-R-Ent strains (24 Escherichia coli) were identified: 25 CTX-M and 3 DHA-1 producers (2 co-producing CTX-Ms). Using the SMS on native stool (“native SMS”) with thresholds ≥60% for both identity and coverage, only 7 of the 23 pre-enriched culture-positive samples resulted positive for blaCTX-M/blaDHA genes (native SMS reads mapping to blaCTX-M/blaDHAs identified in gold-standard: sensitivity, 59.0%; specificity 100%). Moreover, an average of 31.5 and 24.6 antimicrobial resistance genes (ARGs) were detected in the 23 pre-enriched culture-positive and the 19 negative samples, respectively. When the pre-enriched SMS was implemented, more blaCTX-M/blaDHA genes were detected than in the native assay, including in stools that were pre-enriched culture-negative (pre-enriched SMS reads mapping to blaCTX-M/blaDHAs identified in gold-standard: sensitivity, 78.3%; specificity 75.0%). In addition, the pre-enriched SMS identified on average 38.6 ARGs/sample, whereas for the corresponding native SMS it was 29.4 ARGs/sample. Notably, stools resulting false-negative by using the native SMS had lower concentrations of ESC-R-Ent (average: ~105 vs. ~107 CFU/g) and E. coli classified reads (average: 193,959 vs. 1.45 million) than those of native SMS positive samples. Finally, the detection of blaCTX-M/blaDHA genes was compared with two well-established bioinformatic tools. In conclusion, only the pre-enriched SMS assured detection of most carriers of ESC-R-Ent. However, its performance was not comparable to the pre-enriched culture-based approach.

【 授权许可】

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Copyright © 2023 Campos-Madueno, Aldeia, Perreten, Sendi, Moser and Endimiani.