Batch and sampling time exert a larger influence on the fungal community than gastrointestinal location in model animals: A meaningful case study

Frontiers in Nutrition

Nutrition

Jun He¹  Junqiu Luo¹  Bing Yu¹  Zhiqing Huang¹  Jiayan Li¹  Hui Yan¹  Jie Yu¹  Ping Zheng¹  Hua Li¹  Xiangbing Mao¹  Yuheng Luo²  Daiwen Chen² 

[1] Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education of China, Key Laboratory for Animal Disease-Resistance Nutrition and Feed of Ministry of Agriculture of China, Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China;null;

关键词: model animal;    gastrointestinal tract;    fungi;    different batch;    sampling time;   

DOI  :  10.3389/fnut.2022.1021215

 received in 2022-08-17, accepted in 2022-10-11,  发布年份 2022

来源: Frontiers

【 摘 要 】

Fungi play a fundamental role in the intestinal ecosystem and health, but our knowledge of fungal composition and distribution in the whole gastrointestinal tract (GIT) is very limited. The physiological similarity between humans and pigs in terms of digestive and associated metabolic processes places, the pig in a superior position over other non-primate models. Here, we aimed to characterize the diversity and composition of fungi in the GIT of pigs. Using high-throughput sequencing, we evaluated the fungal community in different locations of GIT of 11 pigs with 128.41 ± 1.25 kg body weight acquired successively. Among them, five pigs are sacrificed in April 2019 (Batch 1) and the other six are sacrificed in January 2020 (Batch 2). All subjects with similar genetic backgrounds, housing, management, and diet. Finally, no significant difference is found in the α-diversity (Richness) of the fungal community among all intestinal segments. Basidiomycota and Ascomycota are the two predominant fungal phyla, but Batch 1 harbored a notably high abundance of Basidiomycota and Batch 2 harbored a high abundance of Ascomycota. Moreover, the two batches harbored completely different fungal compositions and core fungal genera. FUNGuild (Fungal Functional Guild) analysis revealed that most of the fungal species present in the GIT are saprotroph, plant pathogen, and animal endosymbiont. Our study is the first to report that even under the same condition, large variations in fungal composition in the host GIT still occur from batch-to-batch and sampling time. The implications of our observations serve as references to the development of better models of the human gut.

【 授权许可】

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Copyright © 2022 Li, Chen, Yu, He, Huang, Zheng, Mao, Li, Yu, Luo, Yan and Luo.