Long-term follow-up of colorectal cancer screening attendees identifies differences in Phascolarctobacterium spp. using 16S rRNA and metagenome sequencing

Frontiers in Oncology

Oncology

Einar Elvbakken Birkeland¹  Vahid Bemanian²  Elina Vinberg³  Geir Hoff⁴  Cecilie Bucher-Johannessen⁵  Trine B. Rounge⁶  Paula Berstad⁷ 

[1] Centre for Bioinformatics, Department of Informatics, University of Oslo, Oslo, Norway;Department of Pathology, Akershus University Hospital, Oslo, Norway;Department of Research, Cancer Registry of Norway, Oslo, Norway;Department of Research, Telemark Hospital Skien, Skien, Norway;Section for Colorectal Cancer Screening, Cancer Registry of Norway, Oslo University Hospital, Oslo, Norway;Department of Tumor Biology, Institute of Cancer Research, Oslo University Hospital, Oslo, Norway;Department of Research, Cancer Registry of Norway, Oslo, Norway;Centre for Bioinformatics, Department of Informatics, University of Oslo, Oslo, Norway;Department of Tumor Biology, Institute of Cancer Research, Oslo University Hospital, Oslo, Norway;Department of Research, Cancer Registry of Norway, Oslo, Norway;Centre for Bioinformatics, Department of Pharmacy, University of Oslo, Oslo, Norway;Section for Colorectal Cancer Screening, Cancer Registry of Norway, Oslo University Hospital, Oslo, Norway;

关键词: archived fecal samples;    colorectal cancer screening;    microbiome;    16S rRNA;    sequencing;    metagenome;    long term follow-up;   

DOI  :  10.3389/fonc.2023.1183039

 received in 2023-03-14, accepted in 2023-03-31,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundThe microbiome has been implicated in the initiation and progression of colorectal cancer (CRC) in cross-sectional studies. However, there is a lack of studies using prospectively collected samples.MethodsFrom the Norwegian Colorectal Cancer Prevention (NORCCAP) trial, we analyzed 144 archived fecal samples from participants who were diagnosed with CRC or high-risk adenoma (HRA) at screening and from participants who remained cancer-free during 17 years of follow-up. We performed 16S rRNA sequencing of all the samples and metagenome sequencing on a subset of 47 samples. Differences in taxonomy and gene content between outcome groups were assessed for alpha and beta diversity and differential abundance.ResultsDiversity and composition analyses showed no significant differences between CRC, HRA, and healthy controls. Phascolarctobacterium succinatutens was more abundant in CRC compared with healthy controls in both the 16S and metagenome data. The abundance of Bifidobacterium and Lachnospiraceae spp. was associated with time to CRC diagnosis.ConclusionUsing a longitudinal study design, we identified three taxa as being potentially associated with CRC. These should be the focus of further studies of microbial changes occurring prior to CRC diagnosis.

【 授权许可】

Unknown   
Copyright © 2023 Bucher-Johannessen, Birkeland, Vinberg, Bemanian, Hoff, Berstad and Rounge