Diabetes with kidney injury may change the abundance and cargo of urinary extracellular vesicles

Frontiers in Endocrinology

Endocrinology

Harry Holthofer¹  Xin Jiang²  Dongfeng Gu²  Yanan Ding²  Beili Shen²  Hequn Zou³  Luca Musante⁴ 

[1] Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;Department of Nephrology and Transplantation Center, Zhengzhou People’s Hospital, Henan University of Chinese Medicine, Zhengzhou, China;Department of Nephrology, South China Hospital of Shenzhen University, Shenzhen, China;Division of Nephrology and Center for Immunity, Inflammation and Regenerative Medicine, Department of Medicine, University of Virginia, Charlottesville, VA, United States;

关键词: urinary extracellular vesicles;    diabetes;    diabetic nephropathy;    diabetes with microalbuminuria;    diabetes with macroproteinuria;   

DOI  :  10.3389/fendo.2023.1085133

 received in 2022-10-31, accepted in 2023-03-03,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundUrinary extracellular vesicles (uEVs) are derived from epithelia facing the renal tubule lumen in the kidney and urogenital tract; they may carry protein biomarkers of renal dysfunction and structural injury. However, there are scarce studies focusing on uEVs in diabetes with kidney injury.Materials and methodsA community-based epidemiological survey was performed, and the participants were randomly selected for our study. uEVs were enriched by dehydrated dialysis method, quantified by Coomassie Bradford protein assay, and adjusted by urinary creatinine (UCr). Then, they identified by transmission electron microscopy (TEM), nanoparticle track analysis (NTA), and western blot of tumor susceptibility gene 101.ResultsDecent uEVs with a homogeneous distribution were finally obtained, presenting a membrane-encapsulated structure like cup-shaped or roundish under TEM, having active Brownian motion, and presenting the main peak between 55 and 110 nm under NTA. The Bradford protein assay showed that the protein concentrations of uEVs were 0.02 ± 0.02, 0.04 ± 0.05, 0.05 ± 0.04, 0.07 ± 0.08, and 0.11 ± 0.15 μg/mg UCr, respectively, in normal controls and in prediabetes, diabetes with normal proteinuria, diabetes with microalbuminuria, and diabetes with macroproteinuria groups after adjusting the protein concentration with UCr by calculating the vesicles-to-creatinine ratio.ConclusionThe protein concentration of uEVs in diabetes with kidney injury increased significantly than the normal controls before and after adjusting the UCr. Therefore, diabetes with kidney injury may change the abundance and cargo of uEVs, which may be involved in the physiological and pathological changes of diabetes.

【 授权许可】

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Copyright © 2023 Gu, Ding, Jiang, Shen, Musante, Holthofer and Zou