Efficacy of a novel bivalent vaccine containing porcine circovirus type 2d and Mycoplasma hyopneumoniae against a dual PCV2d and Mycoplasma hyopneumoniae challenge

Frontiers in Veterinary Science

Veterinary Science

Taehwan Oh¹  Chanhee Chae¹  Jeongmin Suh¹  Sehyeong Ham¹  Byoung-Joo Seo²  Chonghan Kim² 

[1] Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea;WOOGENE B&G CO., LTD., Seoul, Republic of Korea;

关键词: bivalent vaccine;    enzootic pneumonia;    Mycoplasma hyopneumoniae;    porcine circovirus type 2d;    porcine respiratory disease complex;   

DOI  :  10.3389/fvets.2023.1176091

 received in 2023-02-28, accepted in 2023-07-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundInformation on efficacy of a novel bivalent vaccine containing porcine circovirus type 2d (PCV2d) and Mycoplasma hyopneumoniae.ObjectiveTo evaluate bivalent vaccine for efficacy under experimental conditions.AnimalsClinically healthy 35 weaned piglets at 18 days of age were used.MethodsA 2.0 mL dose of bivalent vaccine was administered intramuscularly to pigs at 21 days of age in accordance with the manufacturer’s instructions. The pigs were challenged at 42 days of age either intranasally with PCV2d, or intratracheally with M. hyopneumoniae, or with both.ResultsVaccinated-challenged pigs improved the growth performance compared to pigs that were unvaccinated and then, challenged. Vaccinated-challenged pigs elicited a significant amount of protective immunity for PCV2d-specific neutralizing antibodies and interferon-γ secreting cells (IFN-γ-SC) as well as for M. hyopneumoniae-specific IFN-γ-SC compared to unvaccinated/challenged pigs. Induction of systemic cellular and humoral immune responses from bivalent vaccination reduced the viral and mycoplasmal loads in the blood and larynx. Vaccination and challenge simultaneously reduced both lung and lymphoid lesion severity when compared to unvaccinated-challenged pigs.DiscussionThe results of this study demonstrated that the evaluated bivalent PCV2d and M. hyopneumoniae vaccine was efficacious in protecting pigs from the most predominant PCV2d genotype in the field today, as evaluated with a dual PCV2d and M. hyopneumoniae challenge under experimental conditions.

【 授权许可】

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Copyright © 2023 Ham, Suh, Oh, Kim, Seo and Chae.