Adenoviral-vectored epigraph vaccine elicits robust, durable, and protective immunity against H3 influenza A virus in swine

Frontiers in Immunology

Immunology

Hiep Vu¹  Erika Petro-Turnquist²  Matthew Pekarek²  Nicholas Jeanjaquet²  Eric A. Weaver²  David Steffen³  Cedric Wooledge⁴ 

[1]Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, United States

[2]Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE, United States

[3]Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, United States

[4]School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, United States

[5]Nebraska Veterinary Diagnostic Center, Lincoln, NE, United States

[6]Office of Research and Development, University of Nebraska-Lincoln, Lincoln, NE, United States

关键词: adenoviral (Ad) vector;    epigraph;    swine influenza;    vaccine;    duration;    transmission;    pathology;   

DOI  :  10.3389/fimmu.2023.1143451

 received in 2023-01-13, accepted in 2023-04-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Current methods of vaccination against swine Influenza A Virus (IAV-S) in pigs are infrequently updated, induce strain-specific responses, and have a limited duration of protection. Here, we characterize the onset and duration of adaptive immune responses after vaccination with an adenoviral-vectored Epigraph vaccine. In this longitudinal study we observed robust and durable antibody responses that remained above protective titers six months after vaccination. We further identified stable levels of antigen-specific T cell responses that remained detectable in the absence of antigen stimulation. Antibody isotyping revealed robust class switching from IgM to IgG induced by Epigraph vaccination, while the commercial comparator vaccine failed to induce strong antibody class switching. Swine were challenged six months after initial vaccination, and Epigraph-vaccinated animals demonstrated significant protection from microscopic lesion development in the trachea and lungs, reduced duration of viral shedding, lower presence of infectious virus and viral antigens in the lungs, and significant recall of antigen-specific T cell responses following challenge. The results obtained from this study are useful in determining the kinetics of adaptive immune responses after vaccination with adjuvanted whole inactivated virus vaccines compared to adenoviral vectored vaccines and contribute to the continued efforts of creating a universal IAV-S vaccine.

【 授权许可】

Unknown   
Copyright © 2023 Petro-Turnquist, Pekarek, Jeanjaquet, Wooledge, Steffen, Vu and Weaver