Identification of peptides sequence and conformation contributed to potential allergenicity of main allergens in yogurts

Frontiers in Nutrition

Nutrition

Linbo Shi¹  Meijia Huang²  Fan Yang²  Xin Li³  Xuanyi Meng⁴  Yong Wu⁴  Hongbing Chen⁴ 

[1] School of Basic Medical Sciences, Nanchang University, Nanchang, China;State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China;School of Food Science and Technology, Nanchang University, Nanchang, China;State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China;School of Food Science and Technology, Nanchang University, Nanchang, China;Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang, China;State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China;Sino-German Joint Research Institute (Jiangxi-OAI), Nanchang University, Nanchang, China;Jiangxi Province Key Laboratory of Food Allergy, Nanchang University, Nanchang, China;

关键词: yogurt;    allergen;    allergenicity;    epitope;    peptides;   

DOI  :  10.3389/fnut.2022.1038466

 received in 2022-09-07, accepted in 2022-11-21,  发布年份 2023

来源: Frontiers

【 摘 要 】

Yogurts provide a good source of nutrition and may induce tolerance in people with cow’s milk allergy (CMA). This study aimed to investigate the IgE-binding capacity of main allergens in the different yogurts which provide a reference for people with a high risk of CMA, and analyze the epitopes of major allergen peptides in yogurt. We assessed the degradation and the allergenic properties of major allergens in six commercial yogurts and fresh milk. The degradation of major allergens was analyzed by SDS-PAGE and RP-HPLC. Western blot and ELISA experiments detected allergenic characteristics by using specific sera. The results showed that β-lactoglobulin (Bos d 5) and α-lactalbumin (Bos d 4) were obviously degraded in yogurts but caseins were still present in abundance, which indicated that the proteases in yogurts were specific to whey proteins. IgE and IgG binding ability of major allergens were obviously reduced in yogurts, especially GuMi yogurt. In addition, 17 peptides of major allergens in GuMi yogurt were identified by LC-MS/MS and most of them were located in the interior of the spatial structure of proteins. Among them, 8 peptides had specific biological functions for health benefits, such as antibacterial, antioxidant, and ACE-inhibitory. We also found that 6 and 14 IgE epitopes of Bos d 5 and caseins were destroyed in GuMi yogurt, which could lead to the reduction of IgE-binding capacity. Meanwhile, peptides [Bos d 5 (AA15–40), Bos d 9 (AA120–151, AA125–151)] also preserved T cell epitopes, which might also induce the development of oral tolerance. Therefore, this study suggested that the sequence and conformation of peptides in yogurts contributed to hypoallergenicity.

【 授权许可】

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Copyright © 2023 Huang, Yang, Wu, Meng, Shi, Chen and Li.