Chronic stress boosts systemic inflammation and compromises antiviral innate immunity in Carassius gibel

Frontiers in Immunology

Immunology

Mengke Wu¹  Jianduo Zheng²  Caijiao Dai³  Lijuan Li³  Junfa Yuan⁴  Lin Qi⁵  Ping Deng⁶ 

[1] Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China;Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China;Hubei Engineering Research Center for Aquatic Animal Diseases Control and Prevention, Huazhong Agricultural University, Wuhan, China;Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China;National Aquatic Animal Diseases Para-reference Laboratory, Huazhong Agricultural University (HZUA), Wuhan, China;Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China;National Aquatic Animal Diseases Para-reference Laboratory, Huazhong Agricultural University (HZUA), Wuhan, China;Hubei Engineering Research Center for Aquatic Animal Diseases Control and Prevention, Huazhong Agricultural University, Wuhan, China;Department of Consultation, Tianbin Ruicheng Environmental Technology Engineering Co., LTD, Tianjin, China;Fisheries Science Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan, China;

关键词: inflammation;    chronic stress;    stress;    CyHV-2;    antiviral immune;   

DOI  :  10.3389/fimmu.2023.1105156

 received in 2022-11-23, accepted in 2023-01-26,  发布年份 2023

来源: Frontiers

【 摘 要 】

It is generally considered that stress causes decreased immune function and render fish vulnerable to infection and diseases. However, the molecular mechanisms between stress responses and susceptibility to infections, especially viral diseases, in fish remain unknown. Understanding and monitoring the biological consequences and mechanisms underlying stress responses in fish may contribute to the improvement of animal welfare and production efficiency. In this study, long-term exposure to a variety of stressors, including chasing, overcrowding, restraint stress, and air exposure mimicking chronic stresses, in aquaculture practices was conducted in Carassius gibel to investigate the consequences of chronic stress on inflammation and antiviral capability. With the continuation of stimulation, experimental fish gradually became insensitive to the stress of net chasing and feeding with the accompaniment of upregulated gene expressed in the HPI axis and elevated levels of stress hormones. As expected, stress-induced hyperglycaemia with a decrease in the insulin signaling pathway and altered gene expression in glycolysis and gluconeogenesis, suggesting the disturbance of glycometabolism. Importantly, a link between intestinal homoeostasis and systemic low-grade inflammation in stressed C. gibel was observed, implying crosstalk among the brain, intestine, and other organs. Furthermore, the compromised antiviral capability with impaired antiviral innate immunity in stressed fish was confirmed by RNA sequencing and infection with Cyprinid herpesvirus 2 (CyHV-2), promoting the understanding of enhanced susceptibility to viral infection in stressed fish.

【 授权许可】

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Copyright © 2023 Dai, Zheng, Qi, Deng, Wu, Li and Yuan