The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation

Frontiers in Aging

Aging

Alkmini T. Anastasiadi¹  Issidora S. Papassideri¹  Marianna H. Antonelou¹  Vassilis L. Tzounakas²  Sara Rinalducci³  Veronica Lelli³  Konstantinos Stamoulis⁴  Effie G. Papageorgiou⁵  Anastasios G. Kriebardis⁵ 

[1] Department of Biology, School of Science, National and Kapodistrian University of Athens (NKUA), Athens, Greece;Department of Biology, School of Science, National and Kapodistrian University of Athens (NKUA), Athens, Greece;Department of Biochemistry, School of Medicine, University of Patras, Patras, Greece;Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy;Hellenic National Blood Transfusion Centre, Acharnes, Greece;Laboratory of Reliability and Quality Control in Laboratory Hematology (HemQcR), Department of Biomedical Sciences, School of Health and Welfare Sciences, University of West Attica (UniWA), Egaleo, Greece;

关键词: red blood cell;    glutathione;    oxidative stress;    uric acid;    ascorbic acid;    storage lesion;    accelerated aging;   

DOI  :  10.3389/fragi.2023.1161565

 received in 2023-02-08, accepted in 2023-03-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

Oxidative phenomena are considered to lie at the root of the accelerated senescence observed in red blood cells (RBCs) stored under standard blood bank conditions. It was recently shown that the addition of uric (UA) and/or ascorbic acid (AA) to the preservative medium beneficially impacts the storability features of RBCs related to the handling of pro-oxidant triggers. This study constitutes the next step, aiming to examine the links between hemolysis, redox, and metabolic parameters in control and supplemented RBC units of different storage times. For this purpose, a paired correlation analysis of physiological and metabolism parameters was performed between early, middle, and late storage in each subgroup. Strong and repeated correlations were observed throughout storage in most hemolysis parameters, as well as in reactive oxygen species (ROS) and lipid peroxidation, suggesting that these features constitute donor-signatures, unaffected by the diverse storage solutions. Moreover, during storage, a general “dialogue” was observed between parameters of the same category (e.g., cell fragilities and hemolysis or lipid peroxidation and ROS), highlighting their interdependence. In all groups, extracellular antioxidant capacity, proteasomal activity, and glutathione precursors of preceding time points anticorrelated with oxidative stress lesions of upcoming ones. In the case of supplemented units, factors responsible for glutathione synthesis varied proportionally to the levels of glutathione itself. The current findings support that UA and AA addition reroutes the metabolism to induce glutathione production, and additionally provide mechanistic insight and footing to examine novel storage optimization strategies.

【 授权许可】

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Copyright © 2023 Anastasiadi, Stamoulis, Papageorgiou, Lelli, Rinalducci, Papassideri, Kriebardis, Antonelou and Tzounakas.