Duchenne muscular dystrophy disease severity impacts skeletal muscle progenitor cells systemic delivery

Frontiers in Physiology

Physiology

Corey Switzler¹  Kholoud K. Saleh²  Michael R. Hicks²  Lily Gane²  Devin E. Gibbs³  April D. Pyle³ 

[1] Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA, United States;Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA, United States;Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, CA, United States;Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA, United States;Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, CA, United States;Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA, United States;

关键词: skeletal muscle;    systemic delivery;    duchenne muscular dystrophy;    skeletal muscle progenitor cells;    intra-arterial cell delivery;   

DOI  :  10.3389/fphys.2023.1190524

 received in 2023-03-21, accepted in 2023-04-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

Duchenne muscular dystrophy (DMD) is caused by an out-of-frame mutation in the DMD gene that results in the absence of a functional dystrophin protein, leading to a devastating progressive lethal muscle-wasting disease. Muscle stem cell-based therapy is a promising avenue for improving muscle regeneration. However, despite the efforts to deliver the optimal cell population to multiple muscles most efforts have failed. Here we describe a detailed optimized method of for the delivery of human skeletal muscle progenitor cells (SMPCs) to multiple hindlimb muscles in healthy, dystrophic and severely dystrophic mouse models. We show that systemic delivery is inefficient and is affected by the microenvironment. We found that significantly less human SMPCs were detected in healthy gastrocnemius muscle cross-sections, compared to both dystrophic and severely dystrophic gastrocnemius muscle. Human SMPCs were found to be detected inside blood vessels distinctly in healthy, dystrophic and severely dystrophic muscles, with prominent clotting identified in severely dystrophic muscles after intra arterial (IA) systemic cell delivery. We propose that muscle microenvironment and the severity of muscular dystrophy to an extent impacts the systemic delivery of SMPCs and that overall systemic stem cell delivery is not currently efficient or safe to be used in cell based therapies for DMD. This work extends our understanding of the severe nature of DMD, which should be taken into account when considering stem cell-based systemic delivery platforms.

【 授权许可】

Unknown   
Copyright © 2023 Saleh, Switzler, Hicks, Gane, Gibbs and Pyle.