Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF-mutated colorectal cancer

World Journal of Surgical Oncology

Research

Guiming Chen¹  Jian Qin¹  Tao Jiang¹  Binle Tian²  Qi Li²  Liren Jiang³  Xiaoqin Shi³  Lin Yuan³ 

[1]Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 100 Haining Road, 200080, Shanghai, China

[2]Department of Oncology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 100 Haining Road, 200080, Shanghai, China

[3]Pathology Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 100 Haining Road, 200080, Shanghai, China

关键词: Colorectal cancer;    BRAF;    Microsatellite status;    EBV;    Gallstone disease;   

DOI  :  10.1186/s12957-023-03106-6

 received in 2023-04-23, accepted in 2023-07-12,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundTo investigate the expression of EBV products and frequency of gallstone disease (GD) among different microsatellite status in colorectal cancer (CRC) with BRAFV600E mutation.MethodsWe collected 30 CRC patients with BRAFV600E mutation and 10 BRAF ( −) CRC patients as well as 54 healthy subjects. Tumor tissue samples were collected to detect the mutation of BRAF, KRAS, and TP53. Microsatellite status was determined by immunohistochemistry and PCR. EBER in situ hybridization was performed to detect EBV. In addition, we also collected clinical information about the patients.ResultsWe found that although EBV products were detected in CRC, there were no significant differences in the EBV distribution between the different BRAF groups. Our study demonstrated that BRAFV600E mutation and BRAFV600E with MSI were significantly more frequent in the right CRC. Furthermore, the KRAS mutation rate in the BRAF-wild-type group was proved to be significantly higher than that in the BRAF mutation group. In addition, we revealed that BRAF mutation and MSI were independent risk factors of TNM stage. The frequency of GD was higher in CRC patients than in general population, and although there was no significant difference between CRC with or without BRAFV600E mutation, the highest frequency of GD was found in MSS CRC with BRAFV600E mutation.ConclusionsEBV plays a role in CRC, but is not a determinant of different microsatellite status in CRC with BRAFV600E mutation. The frequency of GD in MSS CRC with BRAFV600E mutation is significantly higher than that in the general population.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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