Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius

BMC Microbiology

Research

Hao Zhang¹  Bo Zhang¹  Peng Shang²  Zhenyu Du³  Jun Xing³  Jing Wang³  Shanfeng Wang³  Jiajun Yang⁴ 

[1] Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China;College of Animal Science, Tibet Agriculture and Animal Husbandry College, 860000, Linzhi, China;School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, 212400, Jurong, Jiangsu, China;School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, 212400, Jurong, Jiangsu, China;Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China;

关键词: Ligilactobacillus salivarius;    Probiotic;    Metabonomic analysis;    Metagenomic analysis;   

DOI  :  10.1186/s12866-023-02993-9

 received in 2023-03-07, accepted in 2023-08-22,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAs substitutes for antibiotics, probiotic bacteria protect against digestive infections caused by pathogenic bacteria. Ligilactobacillus salivarius is a species of native lactobacillus found in both humans and animals. Herein, a swine-derived Ligilactobacillus salivarius was isolated and shown to colonize the ileal mucous membrane, thereby promoting nutritional digestion, absorption, and immunity. To evaluate its probiotic role, the entire genome was sequenced, the genetic information was annotated, and the metabolic information was analyzed.ResultsThe phylogenetic relationship indicated that the bacteria was closer to L. salivarius MT573555.1 and MT585431.1. Functional genes included transporters, membrane proteins, enzymes, heavy metal resistance proteins, and putative proteins; metabolism-related genes were the most abundant. The six types of metabolic pathways secreted by L. salivarius were mainly composed of secretory transmembrane proteins and peptides. The secretory proteins of L. salivarius were digestive enzymes, functional proteins that regulate apoptosis, antibodies, and hormones. Non-targeted metabolomic analysis of L. salivarius metabolites suggested that ceramide, pyrrolidone- 5- carboxylic acid, N2-acetyl-L-ornithine, 2-ethyl-2-hydroxybutyric acid, N-lactoyl-phenylalanine, and 12 others were involved in antioxidation, repair of the cellular membrane, anticonvulsant, hypnosis, and appetite inhibition. Metabolites of clavaminic acid, antibiotic X14889C, and five other types of bacteriocins were identified, namely phenyllactic acid, janthitrem G, 13-demethyl tacrolimus, medinoside E, and tertonasin. The adherence and antioxidation of L. salivarius were also predicted. No virulence genes were found.ConclusionThe main probiotic properties of L. salivarius were identified using genomic, metabonomic, and biochemical assays, which are beneficial for porcine feeding. Our results provided deeper insights into the probiotic effects of L. salivarius.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 7

Fig. 5

Fig. 1

Fig. 1

Fig. 2

Fig. 2

Fig. 7