Proanthocyanidin and sodium butyrate synergistically modulate rat colon carcinogenesis by scavenging free radicals and regulating the COX-2 and APC pathways

The Journal of Basic and Applied Zoology

Research

Hanaa A. Hassan¹  Sameh M. S. El-Halawany²  Elsayed I. Salim²  Ezar H. Hafez² 

[1]Department of Zoology, Faculty of Science, Mansoura University, 35516, Mansoura, Egypt

[2]Research Lab for Molecular Carcinogenesis, Department of Zoology, Faculty of Science, Tanta University, 31527, Tanta, Egypt

关键词: Colon cancer;    Sodium butyrate;    Grape seed proanthocyanidin extract;    Aberrant crypt foci;    APC;    COX-2;    Ki67;    Caspase-3;    Antioxidant activity;   

DOI  :  10.1186/s41936-023-00344-8

 received in 2022-03-23, accepted in 2023-07-21,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe purpose of this study was to explore the effects of sodium butyrate (NaB), grape seed proanthocyanidin extract (GSPE), or their combination against dimethylhydrazine (DMH)-induced aberrant crypt foci (ACF) formation, which is a proxy for colon carcinogenesis in the rat colon.ResultsAfter inducing colon cancer, all treatments markedly decreased the overall numbers of ACF, with the NaB–GSPE combination eliciting the most pronounced reduction. All the treatments significantly inhibited cell proliferation as indicated by the lower percentages of Ki67-positive cells in the colonic mucosa. Also, caspase-3-immunolabeled cells were found to be significantly increased after all treatments, indicating more apoptotic activity in the initiated colonocytes. Further, the treatments significantly modulated the levels of antioxidant biomarkers, including malondialdehyde, superoxide dismutase, reduced glutathione, and total antioxidant capacity, suggesting a potently induced antioxidant activity, especially after the combination treatment. All treatments, especially the combination, dramatically downregulated the expression of COX-2 and APC, both of which are directly linked to colon cancer.ConclusionsNaB and GSPE exert potent anti-carcinogenic effects, both alone but more effectively in combination, in a rat colon cancer model. They could be important for colon cancer treatment and for adjuvant therapy in humans.

【 授权许可】

CC BY   
© Egyptian German Society of Zoology 2023

【 图 表 】

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