Comparative in vivo biodistribution of cells labelled with [89Zr]Zr-(oxinate)4 or [89Zr]Zr-DFO-NCS using PET

EJNMMI Research

Original Research

Mattias Carlsten¹  Li Lu²  Ida Friberger²  Staffan Holmin³  Stefan Milton⁴  Jeroen A. C. M. Goos⁵  Thuy A. Tran⁵  Oscar Ardenfors⁶  Joachim N. Nilsson⁷  Jonathan Siikanen⁸  Erik Samén⁹ 

[1] Center for Hematology and Regenerative Medicine (HERM), Karolinska Institutet, Stockholm, Sweden;Centre for Cell Therapy and Allogeneic Stem Cell Transplantation (CAST), Karolinska Comprehensive Cancer Center, Karolinska University Hospital, Stockholm, Sweden;Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden;Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden;Department of Neuroradiology, Karolinska University Hospital, Stockholm, Sweden;Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden;Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden;Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden;Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden;Department of Radiopharmacy, Karolinska University Hospital, Stockholm, Sweden;Department of Medical Radiation Physics and Nuclear Medicine, Karolinska University Hospital, Stockholm, Sweden;Department of Medical Radiation Physics and Nuclear Medicine, Karolinska University Hospital, Stockholm, Sweden;Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden;Department of Medical Radiation Physics and Nuclear Medicine, Karolinska University Hospital, Stockholm, Sweden;Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden;Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden;Department of Radiopharmacy, Karolinska University Hospital, Stockholm, Sweden;

关键词: Cell tracking;    Zr;    Oxine;    Deferoxamine;    PET;    Dosimetry;    Macrophages;    DSC;   

DOI  :  10.1186/s13550-023-01021-1

 received in 2023-04-27, accepted in 2023-07-18,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundIn vivo monitoring of cell biodistribution using positron emission tomography (PET) provides a quantitative non-invasive method to further optimize cell therapies and related new developments in the field. Our group has earlier optimized and evaluated the in vitro properties of two radiotracers,[89Zr]Zr-(oxinate)4 and [89Zr]Zr-DFO-NCS, for the radiolabelling of different cell types. Here, we performed a microPET study to assess the in vivo biodistribution of cells in rats using these two radiotracers. Human decidual stromal cells (hDSC) and rat macrophages (rMac) were radiolabelled with [89Zr]Zr-(oxinate)4 or [89Zr]Zr-DFO-NCS. Rats were intravenously injected with radiolabelled cells, and the in vivo biodistribution was monitored with microPET/CT imaging for up to day 7. Organ uptake was evaluated and presented as a percentage of injected activity per gram tissue (%IA/g) and total absorbed organ doses (mSv/MBq).ResultsThe biodistribution in vivo showed an immediate uptake in the lungs. Thereafter, [89Zr]Zr-(oxinate)4 labelled cells migrated to the liver, while the signal from [89Zr]Zr-DFO-NCS labelled cells lingered in the lungs. The differences in the in vivo behaviour for the same cell type appeared related to the radiotracer labelling. After 24 h, [89Zr]Zr-(oxinate)4 labelled cells had over 70% higher liver uptake for both hDSC and rMac compared to [89Zr]Zr-DFO-NCS labelled cells, whereas [89Zr]Zr-DFO-NCS labelled cells showed over 60% higher uptake in the lungs compared to [89Zr]Zr-(oxinate)4 labelled cells. This difference in both lung and liver uptake continued until day 7. Dosimetry calculations showed a higher effective dose (mSv/MBq) for [89Zr]Zr-DFO-NCS compared to [89Zr]Zr-(oxinate)4, for both cell types. Although the bone uptake was higher for [89Zr]Zr-(oxinate)4 labelled cells, the prolonged uptake in the lungs contributed to a significant crossfire to bone marrow resulting in a higher bone dose.ConclusionThe [89Zr]Zr-DFO-NCS labelled cells suggest a prolonged accumulation in the lungs, while [89Zr]Zr-(oxinate)4 suggests quicker clearance of the lungs followed by accumulation in the liver. Accumulation of radiolabelled cells in the liver corresponds to other cell-tracking methods. Further studies are required to determine the actual location of the [89Zr]Zr-DFO-NCS labelled cell.

【 授权许可】

CC BY   
© Springer-Verlag GmbH Germany, part of Springer Nature 2023

【 图 表 】

Fig. 2

Fig. 1

Fig. 2

Fig. 1