Determination of serum CA724 levels using fluorescence immunochromatography

BMC Biotechnology

Research

Huankun Liang¹  Shuhai Zhong¹  Laiqing Li¹  Cuicui Chen²  Wenqi Dong³  Chang Liu⁴  Alexander Boronin⁵  Tiancai Liu⁶  Fenglan Peng⁷ 

[1] Guangzhou Youdi Bio-technology Co., Ltd, 510663, Guangzhou, China;Guangzhou Youdi Bio-technology Co., Ltd, 510663, Guangzhou, China;Jinan Laide Bio-technology Co., Ltd, 271100, Jinan, China;Guangzhou Zhenda Biopharmaceutical Technology Co., Ltd, No. 3 Juquan Road, Huangpu District, 510663, Guangzhou, China;Heilongjiang Academy of Chinese Medicine Sciences, 150036, Harbin, China;Laboratory of Plasmid Biology, G.K. Skryabin Institute Of Biochemistry and Physiology of Microorganisms Russian Academy of Sciences, 142290, Pushchino, Russia;School of Laboratory Medicine and Biotechnology, Southern Medical University, 510515, Guangzhou, China;Taian Disabled Soldiers’ Hospital of Shandong Province, 271000, Taian, China;

关键词: CA724;    Biomarker;    Gastric cancer;    Time-resolved fluorescence immunochromatography;    Eu;   

DOI  :  10.1186/s12896-023-00801-w

 received in 2023-04-25, accepted in 2023-08-08,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundCarbohydrate antigen 724 (CA724) is a sensitive and specific indicator for multiple malignant tumors. The aim of this study was to establish a Eu-time resolved fluorescence immunochromatography (Eu-TRFICO) method for quantitative detection of CA724 in serum.MethodsEu-TRFICO strips were optimized and assembled. The sensitivity, specificity and precision were evaluated using CA724 standard dilutions and matrix serum. Meanwhile, the reference interval, comparison, and sensitivity/specificity were performed using clinical negative/positive gastric cancer serum samples.ResultsThe standard curve equation was y = 9.869 x − 154.12 (R2 = 0.993), and the sensitivity was 0.42 U/mL. The common interferents in serum could not affect the quantitative results with low cross-reactivities (all no more than 1.09%). All average recoveries of the intra- and interbatch ranged from 102.38 to 106.40%, and all CVs were below 10%. The reference interval of the healthy subjects was < 4.68 U/mL and the reference interval of the subjects with grade I/II gastric cancer was > 9.54 U/mL. Additionally, a high Pearson r (0.9503) and sensitivity/specificity (92.86%/94.20%) were obtained.ConclusionThis study prepared Eu-TRFICO strips with high sensitivity, specificity, precision and satisfactory clinical testing performance, which provides more options for clinical quantitative and convenient testing of CA724.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

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Fig. 5

Fig. 5