Single-cell sequencing reveals the existence of fetal vascular endothelial stem cell-like cells in mouse liver

Stem Cell Research & Therapy

Research

Tomohiro Iba¹  Hisamichi Naito¹  Hirotaka Konishi²  Fitriana N. Rahmawati²  Shota Shimizu²  Weizhen Jia²  Nobuyuki Takakura³ 

[1] Department of Physiology, School of Medicine, Kanazawa University, Kanazawa, Ishikawa, Japan;Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan;Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan;Immunology Frontier Research Center, Osaka University, Suita, Japan;Center for Infectious Disease Education and Research, Osaka University, Suita, Japan;Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives (OTRI), Osaka University, Suita, Japan;

关键词: Single-cell transcriptomic sequencing;    Endothelial stem cells;    Heterogeneity;    Mouse liver;    Trajectory analysis;    RNA velocity;    Development;   

DOI  :  10.1186/s13287-023-03460-y

 received in 2023-05-18, accepted in 2023-08-21,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundA resident vascular endothelial stem cell (VESC) population expressing CD157 and CD200 has been identified recently in the adult mouse. However, the origin of this population and how it develops has not been characterized, nor has it been determined whether VESC-like cells are present during the perinatal period. Here, we investigated the presence of perinatal VESC-like cells and their relationship with the adult VESC-like cell population.MethodsWe applied single-cell RNA sequencing of endothelial cells (ECs) from embryonic day (E) 14, E18, postnatal day (P) 7, P14, and week (W) 8 liver and investigated transcriptomic changes during liver EC development. We performed flow cytometry, immunofluorescence, colony formation assays, and transplantation assays to validate the presence of and to assess the function of CD157+ and CD200+ ECs in the perinatal period.ResultsWe identified CD200− expressing VESC-like cells in the perinatal period. These cells formed colonies in vitro and had high proliferative ability. The RNA velocity tool and transplantation assay results indicated that the projected fate of this population was toward adult VESC-like cells expressing CD157 and CD200 1 week after birth.ConclusionOur study provides a comprehensive atlas of liver EC development and documents VESC-like cell lineage commitment at single-cell resolution.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 1

40517_2023_266_Article_IEq66.gif

40517_2023_266_Article_IEq19.gif

Fig. 1

Fig. 6

Fig. 16