Genetic-based dissection of resistance to bacterial leaf streak in rice by GWAS

BMC Plant Biology

Research

Lei Chen¹  Yinghua Pan¹  Xueqiang Wang²  Hongliang Zhang³  Xiaoyang Zhu³  Zhanying Zhang³  Jinjie Li³  Zichao Li³ 

[1] Hainan Yazhou Bay Seed Laboratory, 572025, Sanya, Hainan, People’s Republic of China;Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, 530007, Nanning, China;Hainan Yazhou Bay Seed Laboratory, 572025, Sanya, Hainan, People’s Republic of China;Zhejiang Provincial Key Laboratory of Crop Genetic Resources, College of Agriculture and Biotechnology, Zhejiang University, 310058, Hangzhou, China;State Key Laboratory of Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100193, Beijing, China;

关键词: Genome-wide association study;    Rice;    Bacterial leaf streak;    Haplotype analysis;    Transcriptome analysis;   

DOI  :  10.1186/s12870-023-04412-7

 received in 2023-06-28, accepted in 2023-08-14,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundRice is the second-largest food crop in the world and vulnerable to bacterial leaf streak disease. A thorough comprehension of the genetic foundation of agronomic traits was essential for effective implementation of molecular marker-assisted selection.ResultsOur study aimed to evaluate the vulnerability of rice to bacterial leaf streak disease (BLS) induced by the gram-negative bacterium Xanthomonas oryzae pv. oryzicola (Xoc). In order to accomplish this, we first analyzed the population structure of 747 accessions and subsequently assessed their phenotypes 20 days after inoculation with a strain of Xoc, GX01. We conducted genome-wide association studies (GWAS) on a population of 747 rice accessions, consisting of both indica and japonica subpopulations, utilizing phenotypic data on resistance to bacterial leaf streak (RBLS) and sequence data. We identified a total of 20 QTLs associated with RBLS in our analysis. Through the integration of linkage mapping, sequence analysis, haplotype analysis, and transcriptome analysis, we were able to identify five potential candidate genes (OsRBLS1—OsRBLS5) that possess the potential to regulate RBLS in rice. In order to gain a more comprehensive understanding of the genetic mechanism behind resistance to bacterial leaf streak, we conducted tests on these genes in both the indica and japonica subpopulations, ultimately identifying superior haplotypes that suggest the potential utilization of these genes in breeding disease-resistant rice varieties.ConclusionsThe findings of our study broaden our comprehension of the genetic mechanisms underlying RBLS in rice and offer significant insights that can be applied towards genetic improvement and breeding of disease-resistant rice in rapidly evolving environmental conditions.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

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