16S rRNA gene sequencing reveals the effect of fluoxetine on gut microbiota in chronic unpredictable stress-induced depressive-like rats

Annals of General Psychiatry

Research

Wenhuan Xiao¹  Xiaowei Tang¹  Jie Ye¹  Hui Dong¹ 

[1] Teaching hospital of Yangzhou University, Wutaishan Hospital, Yangzhou, Jiangsu Province, China;

关键词: Depression;    Fluoxetine;    Gut microbiota;    Illumina sequencing;    16S rRNA;   

DOI  :  10.1186/s12991-023-00458-x

 received in 2022-07-20, accepted in 2023-07-24,  发布年份 2023

来源: Springer

【 摘 要 】

ObjectivesGut microbiota is relevant to the pathogenesis of mental disorders including depression. This study aimed to investigate the influence of fluoxetine (FLX) on the gut microbiota in rats with Chronic Unpredictable Mild Stresses (CUMS)-induced depression.ResultsWe confirmed that the 28-day CUMS-induced depression rat model. Chronic FLX administration weakly improved depressive-like behaviors in rats. Illumina 16S rRNA gene sequencing on rat feces showed CUMS increased the relative abundance of Firmicutes (60.31% vs. 48.09% in Control, p < 0.05) and Lactobacillus genus (21.06% vs. 6.82% in control, p < 0.05); FLX and CUMS increased Bacilli class (20.00% ~ 24.08% vs. 10.31% in control, p < 0.05).ConclusionCollectively, our study showed that both CUMS and FLX changed the compositions of gut microbiota in rats. FLX and CUMS distinctly regulated the gut microbiota in depressed rats.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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