Advances in single-cell RNA sequencing and its applications in cancer research

Journal of Hematology & Oncology

Review

Xianlan Zhao¹  Qiong Li¹  Jing Xia¹  Dezhi Huang¹  Xinlei Li¹  Xiaoqi Wang¹  Naya Ma¹  Yishuo Duan¹  Bangdong Liu¹  Yang Gou¹  Xi Zhang²  Jun Rao² 

[1] Medical Center of Hematology, Xinqiao Hospital, State Key Laboratory of Trauma, Burn and Combined Injury, Army Medical University, 400037, Chongqing, China;Jinfeng Laboratory, 401329, Chongqing, China;Medical Center of Hematology, Xinqiao Hospital, State Key Laboratory of Trauma, Burn and Combined Injury, Army Medical University, 400037, Chongqing, China;Jinfeng Laboratory, 401329, Chongqing, China;National Clinical Research Center for Hematologic Diseases, the First Affiliated Hospital of Soochow University, 215006, Suzhou, China;

关键词: scRNA-seq;    Cancer;    Heterogeneity;    Tumor microenvironment;    Treatment;   

DOI  :  10.1186/s13045-023-01494-6

 received in 2023-06-09, accepted in 2023-08-09,  发布年份 2023

来源: Springer

【 摘 要 】

Cancers are a group of heterogeneous diseases characterized by the acquisition of functional capabilities during the transition from a normal to a neoplastic state. Powerful experimental and computational tools can be applied to elucidate the mechanisms of occurrence, progression, metastasis, and drug resistance; however, challenges remain. Bulk RNA sequencing techniques only reflect the average gene expression in a sample, making it difficult to understand tumor heterogeneity and the tumor microenvironment. The emergence and development of single-cell RNA sequencing (scRNA-seq) technologies have provided opportunities to understand subtle changes in tumor biology by identifying distinct cell subpopulations, dissecting the tumor microenvironment, and characterizing cellular genomic mutations. Recently, scRNA-seq technology has been increasingly used in cancer studies to explore tumor heterogeneity and the tumor microenvironment, which has increased the understanding of tumorigenesis and evolution. This review summarizes the basic processes and development of scRNA-seq technologies and their increasing applications in cancer research and clinical practice.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 2