A high-resolution genotype–phenotype map identifies the TaSPL17 controlling grain number and size in wheat

Genome Biology

Research

Yan Gong¹  Chenyang Hao¹  Xueyong Zhang¹  Yuanfeng Hao¹  Youzhi Ma¹  Jun Chen¹  Zhonghu He²  Lili Zhang³  Liping Shen³  Danni Liu⁴  Yangyang Liu⁴  Botao Ye⁴  Kuocheng Shen⁴  Xuchang Yu⁴  Ziying Wang⁴  Zifeng Guo⁴  He Wu⁴  Zhiwen Sun⁴  Jianhui Wu⁵  Changbin Yin⁶  Xuebo Zhao⁷  Zhiliang Zhang⁷  Daxing Xu⁷  Lipeng Kang⁷  Song Xu⁷  Aoyue Bi⁷  Fei Lu⁸ 

[1] Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100081, Beijing, China;Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100081, Beijing, China;International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 100081, Beijing, China;Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China;Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China;University of Chinese Academy of Sciences, 100049, Beijing, China;State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, 712100, Yangling, Shaanxi, China;State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, 10011, Beijing, China;University of Chinese Academy of Sciences, 100049, Beijing, China;State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, 10011, Beijing, China;University of Chinese Academy of Sciences, 100049, Beijing, China;State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, 10011, Beijing, China;CAS-JIC Centre of Excellence for Plant and Microbial Science (CEPAMS), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100093, Beijing, China;

关键词: Grain number;    Grain size;    GWAS;    TaSPL17;    Wheat;   

DOI  :  10.1186/s13059-023-03044-2

 received in 2023-04-27, accepted in 2023-08-21,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundLarge-scale genotype–phenotype association studies of crop germplasm are important for identifying alleles associated with favorable traits. The limited number of single-nucleotide polymorphisms (SNPs) in most wheat genome-wide association studies (GWASs) restricts their power to detect marker-trait associations. Additionally, only a few genes regulating grain number per spikelet have been reported due to sensitivity of this trait to variable environments.ResultsWe perform a large-scale GWAS using approximately 40 million filtered SNPs for 27 spike morphology traits. We detect 132,086 significant marker-trait associations and the associated SNP markers are located within 590 associated peaks. We detect additional and stronger peaks by dividing spike morphology into sub-traits relative to GWAS results of spike morphology traits. We propose that the genetic dissection of spike morphology is a powerful strategy to detect signals for grain yield traits in wheat. The GWAS results reveal that TaSPL17 positively controls grain size and number by regulating spikelet and floret meristem development, which in turn leads to enhanced grain yield per plant. The haplotypes at TaSPL17 indicate geographical differentiation, domestication effects, and breeding selection.ConclusionOur study provides valuable resources for genetic improvement of spike morphology and a fast-forward genetic solution for candidate gene detection and cloning in wheat.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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