Chromosomal level genome assembly of medicinal plant Sophora flavescens

Scientific Data

Data Descriptor

Wei Wang¹  Zhipeng Qu²  David L. Adelson³ 

[1] Beijing Zhendong Research Institute, Shanxi Zhendong Pharmaceutical Co Ltd, 10587, Beijing, China;Shanxi Provincial Key Laboratory of Functional Food with Homology of Medicine and Food, Department of Pharmacy, Changzhi Medical College, 046012, Changzhi, China;Zhendong Center, Department of Molecular and Biomedical Sciences, The University of Adelaide, 5005, Adelaide, Australia;Zhendong Center, Department of Molecular and Biomedical Sciences, The University of Adelaide, 5005, Adelaide, Australia;South Australian Museum, 5000, Adelaide, Australia;

关键词: ;   

DOI  :  10.1038/s41597-023-02490-8

 received in 2023-06-02, accepted in 2023-08-18,  发布年份 2023

来源: Springer

【 摘 要 】

Sophora flavescens is a medicinal plant in the genus Sophora of the Fabaceae family. The root of S. flavescens is known in China as Kushen and has a long history of wide use in multiple formulations of Traditional Chinese Medicine (TCM). In this study, we used third-generation Nanopore long-read sequencing technology combined with Hi-C scaffolding technology to de novo assemble the S. flavescens genome. We obtained a chromosomal level high-quality S. flavescens draft genome. The draft genome size is approximately 2.08 Gb, with more than 80% annotated as Transposable Elements (TEs), which have recently and rapidly proliferated. This genome size is ~5x larger than its closest sequenced relative Lupinus albus L. . We annotated 60,485 genes and examined their expression profiles in leaf, stem and root tissues, and also characterised the genes and pathways involved in the biosynthesis of major bioactive compounds, including alkaloids, flavonoids and isoflavonoids. The assembled genome highlights the very different evolutionary trajectories that have occurred in recently diverged Fabaceae, leading to smaller duplicated genomes.

【 授权许可】

CC BY   
© Springer Nature Limited 2023

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