Integrated models of blood protein and metabolite enhance the diagnostic accuracy for Non-Small Cell Lung Cancer

Biomarker Research

Research

Dongqing Wei¹  Chen Zou²  Shijin Xia³  Jiongran Wang⁴  Runhao Xu⁵  Shulin Zhang⁶  Minjie Meng⁷  Qingqing Zhu⁸  Zian Ding⁸  Hao Wang⁸  Zehao Wei⁸  Li Deng⁹  Huimin Wei⁹ 

[1] Department of Bioinformatics, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China;Zhongjing Research and Industrialization Institute of Chinese Medicine, Zhongguancun Scientific Park, 473006, Nanyang, Henan, China;Department of Clinical Laboratory, Children’s Hospital of Shanghai, 200040, Shanghai, China;Department of Geriatrics, Huadong Hospital, Shanghai Institute of Geriatrics, Fudan University, 200040, Shanghai, China;Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China;Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China;Department of Clinical Laboratory, Renji Hospital, 200001, Shanghai, China;Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, 200025, Shanghai, China;Zhongjing Research and Industrialization Institute of Chinese Medicine, Zhongguancun Scientific Park, 473006, Nanyang, Henan, China;Shanghai Public Health Clinical Center, Fudan University, 201508, Shanghai, China;School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, 510006, Guangzhou, China;School of Life Science and Technology, Wuhan Polytechnic University, 430000, Wuhan, China;Shanghai Cellsolution Biotech Co.,Ltd, 200444, Shanghai, China;

关键词: NSCLC;    Blood;    Proteomics;    Metabolite;    Integrated model;   

DOI  :  10.1186/s40364-023-00497-2

 received in 2023-03-29, accepted in 2023-05-05,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundFor early screening and diagnosis of non-small cell lung cancer (NSCLC), a robust model based on plasma proteomics and metabolomics is required for accurate and accessible non-invasive detection. Here we aim to combine TMT-LC-MS/MS and machine-learning algorithms to establish models with high specificity and sensitivity, and summarize a generalized model building scheme.MethodsTMT-LC-MS/MS was used to discover the differentially expressed proteins (DEPs) in the plasma of NSCLC patients. Plasma proteomics-guided metabolites were selected for clinical evaluation in 110 NSCLC patients who were going to receive therapies, 108 benign pulmonary diseases (BPD) patients, and 100 healthy controls (HC). The data were randomly split into training set and test set in a ratio of 80:20. Three supervised learning algorithms were applied to the training set for models fitting. The best performance models were evaluated with the test data set.ResultsDifferential plasma proteomics and metabolic pathways analyses revealed that the majority of DEPs in NSCLC were enriched in the pathways of complement and coagulation cascades, cholesterol and bile acids metabolism. Moreover, 10 DEPs, 14 amino acids, 15 bile acids, as well as 6 classic tumor biomarkers in blood were quantified using clinically validated assays. Finally, we obtained a high-performance screening model using logistic regression algorithm with AUC of 0.96, sensitivity of 92%, and specificity of 89%, and a diagnostic model with AUC of 0.871, sensitivity of 86%, and specificity of 78%. In the test set, the screening model achieved accuracy of 90%, sensitivity of 91%, and specificity of 90%, and the diagnostic model achieved accuracy of 82%, sensitivity of 77%, and specificity of 86%.ConclusionsIntegrated analysis of DEPs, amino acid, and bile acid features based on plasma proteomics-guided metabolite profiling, together with classical tumor biomarkers, provided a much more accurate detection model for screening and differential diagnosis of NSCLC. In addition, this new mathematical modeling based on plasma proteomics-guided metabolite profiling will be used for evaluation of therapeutic efficacy and long-term recurrence prediction of NSCLC.

【 授权许可】

CC BY   
© The Author(s) 2023

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