Diversity of Anopheles species and zoonotic malaria vector of the Buton Utara Wildlife Sanctuary, Southeast Sulawesi, Indonesia

Malaria Journal

Research

Lucia Muslimin¹  Ratmawati Malaka¹  Meyby Eka Putri Lempang²  Farahana Kresno Dewayanti³  Ismail Ekoprayitno Rozi³  Wuryantari Setiadi³  Lepa Syahrani³  Suradi Wangsamuda³  Dendi Hadi Permana³  Puji Budi Setia Asih³  Din Syafruddin⁴ 

[1]Department of Animal Science, Faculty of Animal Husbandry, University of Hasanuddin, Makassar, Indonesia

[2]Doctoral Programme in Medical Science, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia

[3]Eijkman Research Center for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia

[4]Eijkman Research Center for Molecular Biology, National Research and Innovation Agency (BRIN), Cibinong, Indonesia

[5]Department of Parasitology, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia

关键词: Primate malaria;    Anopheles;    Vector molecular identification;   

DOI  :  10.1186/s12936-023-04647-7

 received in 2023-02-23, accepted in 2023-07-13,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe recent deforestation for agricultural, mining, and human re-settlement has significantly reduced the habitat of many non-human primates (NHPs) in Indonesia and intensifies interaction between the NHPs and humans and thus opening the possibility of pathogen spill-over. The emergence of zoonotic malaria, such as Plasmodium knowlesi, poses an immense threat to the current malaria control and elimination that aims for the global elimination of malaria by 2030. As malaria in humans and NHPs is transmitted by the female Anopheles mosquito, malaria vector control is very important to mitigate the spill-over of the malaria parasite to humans. The present study aims to explore the Anopheles species diversity in human settlements adjacent to the wildlife sanctuary forest in Buton Utara Regency, Southeast Sulawesi, Indonesia, and identify the species that potentially transmit the pathogen from monkey to human in the area.MethodsMosquito surveillance was conducted using larval and adult collection, and the collected mosquitoes were identified morphologically and molecularly using the barcoding markers, cytochrome oxidase subunit I (COI), and internal transcribed species 2 (ITS2) genes. Plasmodium sporozoite carriage was conducted on mosquitoes collected through human landing catch (HLC) and human-baited double net trap (HDNT).ResultsThe results revealed several Anopheles species, such as Anopheles flavirostris (16.6%), Anopheles sulawesi (3.3%), Anopheles maculatus (3.3%), Anopheles koliensis (1.2%), and Anopheles vagus (0.4%). Molecular analysis of the sporozoite carriage using the primate-specific malaria primers identified An. sulawesi, a member of the Leucosphyrus group, carrying Plasmodium inui sporozoite.ConclusionsThis study indicates that the transmission of zoonotic malaria in the area is possible and alerts to the need for mitigation efforts through a locally-tailored vector control intervention and NHPs habitat conservation.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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