Functional ProTracer identifies patterns of cell proliferation in tissues and underlying regulatory mechanisms

npj Regenerative Medicine

Brief Communication

Qing-Dong Wang¹  Yulong He²  Xufeng Li³  Kuo Liu³  Xiuxiu Liu⁴  Wenjuan Pu⁴  Wendong Weng⁴  Maoying Han⁴  Bin Zhou⁵  Yan Li⁶  Jan S. Tchorz⁷  Ruling Shen⁸  Ruilin Sun⁹  Yi-Han Chen^1,10  Dandan Liang^1,10  Lingjuan He^1,11 

[1] Bioscience Cardiovascular, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden;Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Cam-Su Genomic Resources Center, Soochow University, Suzhou, China;Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China;New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China;New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China;Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China;School of Life Science and Technology, ShanghaiTech University, Shanghai, China;New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China;Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai, Shandong, China;State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China;Novartis Institutes for BioMedical Research, Novartis Pharma AG, Basel, Switzerland;Shanghai Laboratory Animal Research Center, Shanghai, China;Shanghai Model Organisms Center, Inc., Shanghai, China;State Key Laboratory of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China;Westlake University School of Life Sciences, Hangzhou, China;

关键词: ;   

DOI  :  10.1038/s41536-023-00318-y

 received in 2023-03-30, accepted in 2023-07-21,  发布年份 2023

来源: Springer

【 摘 要 】

A genetic system, ProTracer, has been recently developed to record cell proliferation in vivo. However, the ProTracer is initiated by an infrequently used recombinase Dre, which limits its broad application for functional studies employing floxed gene alleles. Here we generated Cre-activated functional ProTracer (fProTracer) mice, which enable simultaneous recording of cell proliferation and tissue-specific gene deletion, facilitating broad functional analysis of cell proliferation by any Cre driver.

【 授权许可】

CC BY   
© Springer Nature Limited 2023

【 图 表 】

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Fig. 1