Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot

BMC Plant Biology

Research

Quan-Hong Yao¹  Ai-Sheng Xiong²  Ya-Hui Wang²  Yuan-Jie Deng²  Bo Wang³ 

[1] Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Science, 201106, Shanghai, China;State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China;State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China;Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Science, 201106, Shanghai, China;

关键词: Genetically modified carrot;    Betanin;    Unintended effects;    Photosynthesis;    Transcriptome;    Metabolomics;   

DOI  :  10.1186/s12870-023-04383-9

 received in 2022-09-12, accepted in 2023-07-14,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundBetalain is a natural pigment with important nutritional value and broad application prospects. Previously, we produced betanin biosynthesis transgenic carrots via expressing optimized genes CYP76AD1S, cDOPA5GTS and DODA1S. Betanin can accumulate throughout the whole transgenic carrots. But the effects of betanin accumulation on the metabolism of transgenic plants and whether it produces unexpected effects are still unclear.ResultsThe accumulation of betanin in leaves can significantly improve its antioxidant capacity and induce a decrease of chlorophyll content. Transcriptome and metabolomics analysis showed that 14.0% of genes and 33.1% of metabolites were significantly different, and metabolic pathways related to photosynthesis and tyrosine metabolism were markedly altered. Combined analysis showed that phenylpropane biosynthesis pathway significantly enriched the differentially expressed genes and significantly altered metabolites.ConclusionsResults showed that the metabolic status was significantly altered between transgenic and non-transgenic carrots, especially the photosynthesis and tyrosine metabolism. The extra consumption of tyrosine and accumulation of betanin might be the leading causes.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 3

Fig. 1

Fig. 3

13690_2023_1147_Article_IEq6.gif

Fig. 1

Fig. 1