The role of cortisol in immunosuppression in subarachnoid haemorrhage

European Journal of Medical Research

Research

Navneet Singh¹  Sharon Hulme²  Andrew King²  Pippa Tyrrell²  David O. Ajao³  Nancy Rothwell⁴  James Galea⁵  Stephen J. Hopkins⁶  Margaret E. Hoadley⁶ 

[1] Atkinson Morley, Dept of Neurosurgery, St Georges Hospital, Blackshaw Rd, Sw17 0QT, London, UK;Division of Cardiovascular Sciences, Manchester Academic Health Sciences Centre, University of Manchester, Stott Lane, M13 9PT, Salford, UK;Division of Cardiovascular Sciences, Manchester Academic Health Sciences Centre, University of Manchester, Stott Lane, M13 9PT, Salford, UK;Stockport NHS Foundation Trust, Stepping Hill Hospital, Poplar Grove, Hazel Grove, SK2 7JE, Stockport, UK;Faculty of Life Sciences, University of Manchester, AV Hill Building, Oxford Road, M13 9PL, Manchester, UK;Ninewells Hospital and Medical School, University of Dundee, DD1 9SY, Ninewells, Dundee, UK;Division of Cardiovascular Sciences, Manchester Academic Health Sciences Centre, University of Manchester, Stott Lane, M13 9PT, Salford, UK;Northern Care Alliance Research and Innovation, Salford Royal NHS Foundation Trust, Stott Lane, M6 8HD, Salford, UK;Division of Cardiovascular Sciences, Manchester Academic Health Sciences Centre, University of Manchester, Stott Lane, M13 9PT, Salford, UK;

关键词: Cytokines;    Immune response;    Immunosuppression;    Cortisol;    RU-486;    Infection;   

DOI  :  10.1186/s40001-023-01222-3

 received in 2022-09-21, accepted in 2023-07-12,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundWe sought to determine the extent to which cortisol suppressed innate and T cell-mediated cytokine production and whether it could be involved in reducing peripheral cytokine production following subarachnoid haemorrhage (SAH).MethodsWhole blood from healthy controls, patients with SAH and healthy volunteers was stimulated with lipopolysaccharide (LPS), to stimulate innate immunity, or phytohaemagglutinin (PHA), to stimulate T cell-mediated immunity. Varying concentrations of cortisol were included, with or without the cortisol antagonist RU486. Concentration of interleukin-6 (IL-6), IL-1β and tumour necrosis factor-alpha) TNFα were determined as a measure of innate immunity. IL-6, IL-17 (interferon gamma) IFNƔ and IL-17 were determined as an indicator of T cell-mediated immunity.ResultsSuppression of innate responses to LPS was apparent in whole blood from SAH patients, relative to healthy controls, and TNFα production was inversely correlated with plasma cortisol concentration. Cytokine production in whole blood from healthy volunteers was inhibited by cortisol concentrations from 0.33 µM, or 1 µM and above, and these responses were effectively reversed by the cortisol antagonist RU-486. In SAH patients, RU-486 reversed suppression of innate TNF-α and IL-6 responses, but not IL-1ß or T cell-mediated responses.ConclusionThese data suggest that cortisol may play a role in reducing innate, but not T cell-mediated immune responses in patients with injuries such as SAH and that cortisol antagonists could be effective in boosting early innate responses.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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