Identification and characterization of a polyomavirus in the thornback skate (Raja clavata)

Virology Journal

Brief Report

Inês Farias¹  Fabiana Neves²  Ana Veríssimo²  Paulo Pereira²  Joana Abrantes³  Catarina Maia⁴  Arvind Varsani⁵ 

[1] CIBIO-InBIO, Research Center in Biodiversity and Genetic Resources, University of Porto, 4485-661, Vairão, Portugal;CIBIO-InBIO, Research Center in Biodiversity and Genetic Resources, University of Porto, 4485-661, Vairão, Portugal;BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, 4485-661, Vairão, Portugal;CIBIO-InBIO, Research Center in Biodiversity and Genetic Resources, University of Porto, 4485-661, Vairão, Portugal;BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, 4485-661, Vairão, Portugal;Department of Biology, Faculty of Sciences, University of Porto, 4169-007, Porto, Portugal;Portuguese Institute for Sea and Atmosphere, Division of Modelling and Management of Fisheries Resources, 1495-165, Alges, Portugal;The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ, USA;Structural Biology Research Unit, Department of Integrative, Biomedical Sciences, University of Cape Town, 7925, Observatory, Cape Town, South Africa;

关键词: Cartilaginous fishes;    Thornback skate;    Polyomaviridae;   

DOI  :  10.1186/s12985-023-02149-1

 received in 2023-07-06, accepted in 2023-08-02,  发布年份 2023

来源: Springer

【 摘 要 】

Members of the family Polyomaviridae have a circular double-stranded DNA genome that have been identified in various hosts ranging from mammals to arachnids. Here we report the identification and analysis of a complete genome sequence of a novel polyomavirus, Raja clavata polyomavirus (RcPyV1), from a cartilaginous fish, the thornback skate (Raja clavata). The genome sequence was determined using a metagenomics approach with an aim to provide baseline viral data in cartilaginous fish in different ecosystems. The RcPyV1 genome (4,195 nucleotides) had typical organization of polyomavirus, including early antigens (small T; Large T) encoded on one strand and late viral proteins (VP1; VP2) on the complementary strand. Maximum-likelihood phylogenetic analysis of the large T-antigen revealed that RcPyV1 clusters with a polyomavirus obtained from another cartilaginous fish, the guitarfish polyomavirus 1 (GfPyV1). These two share ~ 56% pairwise identity in LT and VP1 protein sequences. These analyses support the hypothesis that cartilaginous fishes have a specific lineage of polyomaviruses.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 1

Fig. 1