Dysbiosis of gut microbiota during fecal stream diversion in patients with colorectal cancer

Gut Pathogens

Research

Soo Young Lee¹  Hyeong Rok Kim¹  Hyeung-Min Park¹  Chang Hyun Kim¹ 

[1] Department of Surgery, Chonnam National University Hwasun Hospital and Medical School, 322 Seoyang-Ro Hwasun-Eup, 58128, Hwasun-Gun, Jeonnam, South Korea;

关键词: Fecal stream diversion;    Microbiota;    Colorectal cancer;    Ileostomy;    Dysbiosis;   

DOI  :  10.1186/s13099-023-00566-9

 received in 2023-06-20, accepted in 2023-08-10,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe effect of fecal stream diversion on the gut microbiota is still uncertain. The present study was designed to assess the effect of fecal stream diversion on the composition of the gut microbiota in patients with colorectal cancer. We included patients undergoing left-sided colorectal cancer surgery with (ileostomy group) or without (control group) diverting ileostomy. Fecal samples were collected from 10 patients in each group before surgery (t1) and after ileostomy repair in the ileostomy group and 6–12 months after the initial surgery in the control group (t2). The fecal microbiota was assessed using 16S rRNA sequencing, and changes in the composition of the fecal microbiota were compared between the two groups.ResultsAlpha diversity analysis revealed that the complexity of fecal microbiota decreased between t1 and t2 only in the ileostomy group. Beta diversity analysis also showed dissimilarity between t1 and t2 only in the ileostomy group. The composition of the microbiota was similar between the two groups at t1. However, at t2, the ileostomy group had lower proportion of beneficial bacteria (Lachnospiraceae, 3.8% vs. 29.9%, p < 0.001; Ruminococcaceae, 0.6% vs. 18.4%, p < 0.001; Blautia, 0.1% vs. 9.1%, p < 0.001; Faecalibacterium, 0.2% vs. 7.5%, p < 0.001) and a higher proportion of harmful bacteria (Proteobacteria, 17.9% vs. 5.1%, p = 0.006; Clostridium, 16.2% vs. 1.1%, p = 0.013; Streptococcus, 17.7% vs. 1.6%, p = 0.002) than the control group.ConclusionsFecal stream diversion was closely associated with less diversity and dysbiosis of the gut microbiota.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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